Publication: Apoptosis and antimigration induction in human skin cancer cells by rhodomyrtone
1
0
Issued Date
2018
Resource Type
File Type
application/pdf
ISSN
17920981
Other identifier(s)
2-s2.0-85046544304
Rights Holder(s)
Scopus
Bibliographic Citation
Experimental and Therapeutic Medicine. Vol 15, No.6 (2018), p.5035-5040
Suggested Citation
Tayeh M., Nilwarangkoon S., Tanunyutthawongse C., Mahabusarakum W., Watanapokasin R. Apoptosis and antimigration induction in human skin cancer cells by rhodomyrtone. Experimental and Therapeutic Medicine. Vol 15, No.6 (2018), p.5035-5040. doi:10.3892/etm.2018.6044 Retrieved from: https://hdl.handle.net/20.500.14740/4246
Abstract
Rhodomyrtone is a bioactive compound extracted from Rhodomyrtus tomentosa leaves. It has been used as a traditional herb medicine for many years. Rhodomyrtone exhibits antibacterial activity, anti-inflammatory and antioxidant activities. However, the anticancer activity of rhodomyrtone has not been previously reported. The present study investigated the anticancer effect of rhomyrtone on human epidermoid carcinoma A431 cells. The cytotoxic and antiproliferative effects of rhodomyrtone on A431 cells were investigated by an MTT assay. Cell morphological alterations and apoptotic cells were observed with Hoechst 33342 staining following rhodomyrtone treatment. Flow cytometry and western blotting were performed to detect cell cycle and apoptosis induction. The results demonstrated that rhodomyrtone inhibited proliferation of A431 cells in a dose-dependent manner with IC50 value of 8.04±0.11 µg/ml. The results also indicated that rhodomyrtone increased chromatin condensation, nuclear fragmentation and apoptotic bodies in treated A431 cells in a time-dependent manner. Apoptosis was also induced through the activation of caspase-7 and poly (ADP-Ribose) polymerase cleavage. Flow cytometry analysis revealed that rhodomyrtone induced cell cycle arrest at the G1 phase. Notably, the non-toxic concentration of rhodomyrtone markedly inhibited A431 cell migration in a dose- and time-dependent manner. These finding suggested that rhodomyrtone may be used as an anticancer agent for human skin cancer. © 2018, Spandidos Publications. All rights reserved.
Subject(s)
Caspase 7
Nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase
Plant medicinal product
Rhodomyrtone
Unclassified drug
Antineoplastic activity
Apoptosis
Article
Cell migration
Cell proliferation
Chromatin condensation
Controlled study
Flow cytometry
Fluorescence microscopy
G1 phase cell cycle checkpoint
Growth inhibition
Human
Human cell
IC50
MTT assay
Skin cancer
Western blotting
Wound healing
Nicotinamide adenine dinucleotide adenosine diphosphate ribosyltransferase
Plant medicinal product
Rhodomyrtone
Unclassified drug
Antineoplastic activity
Apoptosis
Article
Cell migration
Cell proliferation
Chromatin condensation
Controlled study
Flow cytometry
Fluorescence microscopy
G1 phase cell cycle checkpoint
Growth inhibition
Human
Human cell
IC50
MTT assay
Skin cancer
Western blotting
Wound healing