Publication:
Spermidine-induced apoptosis via reactive oxygen species generation and caspase 3 activation in mouse P19 embryonal carcinoma cells

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Issued Date

2010

Resource Type

Article

File Type

application/pdf

ISSN

18117775

DOI

10.3923/ijp.2010.903.909

Other identifier(s)

2-s2.0-77958029206

Rights Holder(s)

Scopus

Bibliographic Citation

International Journal of Pharmacology. Vol 6, No.6 (2010), p.903-909

Suggested Citation

Nusuetrong P., Suwannasual U., Meksuriyen D. Spermidine-induced apoptosis via reactive oxygen species generation and caspase 3 activation in mouse P19 embryonal carcinoma cells. International Journal of Pharmacology. Vol 6, No.6 (2010), p.903-909. doi:10.3923/ijp.2010.903.909 Retrieved from: https://hdl.handle.net/20.500.14740/7672

Title

Spermidine-induced apoptosis via reactive oxygen species generation and caspase 3 activation in mouse P19 embryonal carcinoma cells

Author(s)

Nusuetrong P.
 Suwannasual U.
 Meksuriyen D.

Abstract

The present study was to investigate the effects of spermidine-induced apoptosis in mouse PI 9 embryonal carcinoma cells. The cell viability using MTT (3-[4,5-dimefhyl-2-thiazolyl]-2,5-diphenyl-2H-tetrazolium bromide) assay showed that spermidine decreased cell viability after 24-h incubation in a concentration-dependent manner with an IC50 value of approximately 20 μM. Using Hoechst33342 staining, chromatin condensation and apoptotic bodies in PI 9 cells treated with spermidine were observed. Moreover, flow cytometric analysis stained with propidium iodide increased the accumulation of DNA fragmentation in the pre GJGi phase, indicating apoptotic cell death, which was accompanied by the generation of reactive oxygen species (ROS) as detected with the fluorescence probe 2',7'-dichlorofluorescin diacetate (DCFH-DA). Western blot analysis revealed that spermidine-induced apoptosis was associated with an increase of Bax and caspase 3 expression, meanwhile the expression of Bcl-2 was decreased. The results suggest that spermidine-induced PI 9 apoptotic cell death is involving ROS, Bcl-2 family as well as caspase 3. © 2010 Asian Network for Scientific Information.

Subject(s)

3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide
 Caspase 3
 Dichlorodihydrofluorescein diacetate
 Hoe 33342
 Propidium iodide
 Protein Bax
 Protein bcl 2
 Reactive oxygen metabolite
 Spermidine
 Animal cell
 Apoptosis
 Article
 Cell cycle G0 phase
 Cell cycle G1 phase
 Cell viability
 Chromatin condensation
 Controlled study
 Cytotoxicity test
 DNA fragmentation
 Down regulation
 Embryo
 Embryonal carcinoma stem cell
 Enzyme activation
 Flow cytometry
 Fluorescence analysis
 Incubation time
 Mouse
 Nonhuman
 Protein expression
 Staining
 Toxicity testing
 Upregulation
 Western blotting

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https://hdl.handle.net/20.500.14740/7672

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