Publication: Extracellular Matrix Proximity Biotinylation Identifies Periostin as a PHEX Proteolytic Substrate
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Issued Date
2025-06-30
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ISSN
08926638
eISSN
15306860
Scopus ID
2-s2.0-105008734960
Journal Title
FASEB Journal
Volume
39
Issue
12
Rights Holder(s)
SCOPUS
Bibliographic Citation
FASEB Journal Vol.39 No.12 (2025)
Suggested Citation
Dusadeemeelap C., Matsubara T., Kokabu S., Addison W.N. Extracellular Matrix Proximity Biotinylation Identifies Periostin as a PHEX Proteolytic Substrate. FASEB Journal Vol.39 No.12 (2025). doi:10.1096/fj.202500635RR Retrieved from: https://hdl.handle.net/20.500.14740/21131
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Abstract
Inactivating mutations in the PHEX gene lead to X-linked hypophosphatemia (XLH), which is characterized by impaired skeletal mineralization and low serum phosphate. Subsequent rickets and osteomalacia result in bone deformities and pseudofractures. A hallmark of XLH is an intrinsic defect in osteoblast function resulting in altered bone matrix composition typified by the local accumulation of extracellular matrix proteins and peptide fragments. PHEX is a membrane-bound endopeptidase expressed in osteoblasts and osteocytes. Little is known about PHEX proteolytic substrates or the protein–protein interactions governing PHEX function. Classical affinity purification approaches are challenging in studies of the extracellular environment. Here, we developed an approach for unbiased identification of the extracellular proximal interactome of PHEX in osteoblasts using proximity-dependent biotin identification combined with affinity purification and mass spectrometry. By tagging the PHEX extracellular domain with BioID2 biotin ligase, we labeled and unveiled a PHEX proximity network consisting of 39 high-confidence proteins. Notably, several candidates with documented roles in bone morphogenesis and matrix organization were identified. We validated interaction of PHEX with periostin, a bone-matrix protein associated with collagen-fibril organization, cell adhesion and cell migration. Co-transfection experiments and cell-free enzyme cleavage assays revealed proteolytic cleavage of secreted periostin by PHEX. In conclusion, BioID2 is a powerful strategy to explore cell-matrix relationships in osteoblasts. These results present a novel map of the PHEX interactome and serve as a valuable resource for unraveling the mechanisms underlying PHEX function and XLH.