Publication:
Wound healing activity of Pluchea indica leaf extract in oral mucosal cell line and oral spray formulation containing nanoparticles of the extract

1
0

Issued Date

2017

Resource Type

Article

File Type

application/pdf

ISSN

13880209

DOI

10.1080/13880209.2017.1326511

Other identifier(s)

2-s2.0-85026777680

Rights Holder(s)

Scopus

Bibliographic Citation

Pharmaceutical Biology. Vol 55, No.1 (2017), p.1767-1774

Suggested Citation

Buranasukhon W., Athikomkulchai S., Tadtong S., Chittasupho C. Wound healing activity of Pluchea indica leaf extract in oral mucosal cell line and oral spray formulation containing nanoparticles of the extract. Pharmaceutical Biology. Vol 55, No.1 (2017), p.1767-1774. doi:10.1080/13880209.2017.1326511 Retrieved from: https://hdl.handle.net/20.500.14740/4674

Title

Wound healing activity of Pluchea indica leaf extract in oral mucosal cell line and oral spray formulation containing nanoparticles of the extract

Author(s)

Buranasukhon W.
 Athikomkulchai S.
 Tadtong S.
 Chittasupho C.

Abstract

Context: Pluchea indica (L.) Less (Asteraceae) is an herb used as a traditional medicine for wound healing. The chemical compounds found in Pluchea indica leaves are phenolic acids, flavonoids, anthocyanins and carotenoids. Objective: This study investigates the effect of Pluchea indica leaf ethanol extract and its nanoparticles (NPs) on cytotoxicity, cell survival and migration of human oral squamous carcinoma cell line. Materials and methods: Cell viability was measured using MTT assay to assess the effect of Pluchea indica leaf extract and NPs (1–500 µg/mL) on cytotoxicity and cell survival. The effect of Pluchea indica leaf extract and NPs on cell migration was determined by scratch assay. The % relative migration was calculated after 24, 48 and 72 h of treatment. Results: The sizes of Pluchea indica leaf extract NPs were in a range of nanometers. NPs possessed negative charge with the polydispersity index (PDI) smaller than 0.3. After the treatment for 24, 48 and 72 h, Pluchea indica leaf extract had IC50 value of 443.2, 350.9 and 580.5 µg/mL, respectively, whereas the IC50 value of NPs after the treatment for 24, 48 and 72 h were 177.4, 149.2 and 185.1 µg/mL, respectively. The % relative migration of cells was significantly increased when the cells were treated with 62.5 and 125 µg/mL of the extract and 62.5 µg/mL of NPs. Discussion and Conclusions: NPs increased cytotoxicity of the Pluchea indica leaf extract, increased the migration of cells at low concentration and increased colloidal stability of the extract in an oral spray formulation. © 2017 The Author(s).

Subject(s)

Alcohol
 Nanoparticle
 Plant extract
 Pluchea indica extract
 Unclassified drug
 Wound healing promoting agent
 Plant extract
 Solvent
 Article
 Carcinoma cell line
 Cell migration
 Cell survival
 Concentration response
 Controlled study
 Dispersity
 Drug cytotoxicity
 Drug dosage form comparison
 Drug effect
 Drug formulation
 Drug stability
 Human
 Human cell
 IC50
 Microbiological stability
 Mouth carcinoma
 MTT assay
 Nonhuman
 Oral squamous carcinoma cell line
 Particle size
 Plant leaf
 Pluchea indica
 Wound healing
 Wound healing assay
 Aerosol
 Asteraceae
 Cell motion
 Chemistry
 Dose response
 Drug effects
 Isolation and purification
 Medicinal plant
 Mouth mucosa
 Pathology
 Phytotherapy
 Stomatitis
 Time factor
 Tumor cell line
 Wound healing
 Aerosols
 Asteraceae
 Cell Line, Tumor
 Cell Movement
 Cell Survival
 Dose-Response Relationship, Drug
 Drug Compounding
 Drug Stability
 Ethanol
 Humans
 Inhibitory Concentration 50
 Mouth Mucosa
 Nanoparticles
 Phytotherapy
 Plant Extracts
 Plant Leaves
 Plants, Medicinal
 Solvents
 Stomatitis
 Time Factors
 Wound Healing

View online Resources

Research Projects

Organizational Units

Journal Issue

URI

https://hdl.handle.net/20.500.14740/4674

Collections

Scopus: Year 1983-2021

Endorsement

Review

Supplemented By

Referenced By