Publication:
Apoptotic effects of satratoxin H is mediated through DNA double-stranded break in PC12 cells

0
0

Issued Date

2012

Resource Type

Article

File Type

application/pdf

ISSN

18803989

DOI

10.2131/jts.37.803

Other identifier(s)

2-s2.0-84865636333

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of Toxicological Sciences. Vol 37, No.4 (2012), p.803-812

Suggested Citation

Nusuetrong P., Saito M., Kikuchi H., Oshima Y., Moriya T., Nakahata N. Apoptotic effects of satratoxin H is mediated through DNA double-stranded break in PC12 cells. Journal of Toxicological Sciences. Vol 37, No.4 (2012), p.803-812. doi:10.2131/jts.37.803 Retrieved from: https://hdl.handle.net/20.500.14740/6974

Title

Apoptotic effects of satratoxin H is mediated through DNA double-stranded break in PC12 cells

Author(s)

Nusuetrong P.
 Saito M.
 Kikuchi H.
 Oshima Y.
 Moriya T.
 Nakahata N.

Abstract

Satratoxin H is an important air- and food-borne mycotoxin, which has been implicated in human health damage. Satratoxin H is known to induce apoptosis as well as genotoxicity in PC12 cells. In the present study, we further investigated the mechanism of apoptotic effects of satratoxin H with focus on caspase-3 and poly-ADP-ribose polymerase (PARP) pathway. We also examined whether it induces DNA damage in PC12 cells. In the cells treated with satratoxin H, caspase-3 was cleaved in a time-dependent manner. Furthermore, satratoxin H induced cleavage of PARP, one of the downstream molecules of caspase-3. The cleavage was inhibited by SB203580, a p38 MAPK inhibitor, or SP600125, a JNK inhibitor. Satratoxin H, however, had no effect on expression levels of Bax and Bcl-2. Furthermore, the micronucleus assay revealed that satratoxin H induced chromosome break. Also, satratoxin H increased the level of phosphorylation of histone H2A, indicating that it caused DNA double-stranded breaks in PC12 cells. Meanwhile, no genotoxicity was detected with any of treatments carried out in the alkaline comet assay. These results imply that satratoxin H induces genotoxicity by DNA double-stranded break. Our results suggest a considerable potential for the genotoxic risk associated with the presence of satratoxin H.

Subject(s)

4 (4 fluorophenyl) 2 (4 methylsulfinylphenyl) 5 (4 pyridyl)imidazole
 Anthra[1,9 cd]pyrazol 6(2h) one
 Caspase 3
 Double stranded DNA
 Histone H2A
 Mycotoxin
 Protein Bax
 Protein bcl 2
 Satratoxin h
 Unclassified drug
 Animal cell
 Apoptosis
 Article
 Cell strain
 Chromosome breakage
 Controlled study
 DNA strand breakage
 Genotoxicity
 Micronucleus test
 Nonhuman
 Protein blood level
 Protein cleavage
 Protein expression
 Rat
 Animals
 Anthracenes
 Apoptosis
 Bcl-2-Associated X Protein
 Blotting, Western
 Caspase 3
 Comet Assay
 DNA Breaks, Double-Stranded
 DNA Damage
 JNK Mitogen-Activated Protein Kinases
 P38 Mitogen-Activated Protein Kinases
 PC12 Cells
 Phosphorylation
 Poly(ADP-ribose) Polymerases
 Rats
 Trichothecenes
 Stachybotrys chartarum

View online Resources

Research Projects

Organizational Units

Journal Issue

URI

https://hdl.handle.net/20.500.14740/6974

Collections

Scopus: Year 1983-2021

Endorsement

Review

Supplemented By

Referenced By