Publication: The inhibitory effect of 13-butoxyberberine bromide on migration in breast cancer MDA-MB-231 cells
7
0
Issued Date
2019
Resource Type
File Type
application/pdf
ISSN
1252208
Other identifier(s)
2-s2.0-85070210485
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
Journal of the Medical Association of Thailand. Vol 102, No.7 (2019), p.12-16
Suggested Citation
Laomethakorn P., Jaitrong M., Samosorn S., Watanapokasin R. The inhibitory effect of 13-butoxyberberine bromide on migration in breast cancer MDA-MB-231 cells. Journal of the Medical Association of Thailand. Vol 102, No.7 (2019), p.12-16. Retrieved from: https://hdl.handle.net/20.500.14740/5219
Abstract
Background: 13-butoxyberberine bromide is a berberine derivative that has not been reported on the anti-migration mechanism of breast cancer. Cancer metastasis is the major cause of cancer mortality, about 90% of cancer patients death are caused by cancer metastasis not the primary tumor. Therefore, the compound that could inhibit cancer migration is necessary for development as anti-cancer drug. Objective: To investigate the effects of 13-butoxyberberine bromide on the anti-migration of breast cancer MDA-MB-231 cells. Materials and Methods: Cell viability was determined by MTT assay. Wound-healing assay and transwell chamber assay were used to determine cell migration. Protein expression levels were examined by western blot analysis. Results: 13-Butoxyberberine bromide at sub-toxic concentration (10 μg/mL) inhibited cell migration in MDA-MB-231 treated cells via down-regulation of MMP-2 and MMP-9 expression which play important role in cancer cell metastasis. Conclusion: 13-Butoxyberberine bromide at 10 μg/mL showed anti-migration effect against MDA-MB-231 cells through inhibition of mediator proteins. These findings could be useful in the development of 13-butoxyberberine bromide to be a novel anti-migration agent for clinical use in the future. © Journal Of The Medical Association Of Thailand| 2019.
Subject(s)
13 butoxyberberine bromide
Berberine derivative
Beta actin
Chemoattractant
Gelatinase A
Gelatinase B
Proteinase inhibitor
Unclassified drug
Article
Breast cancer
Cell migration
Cell migration assay
Cell survival
Cell viability
Chemoluminescence
Controlled study
Down regulation
Human
Human cell
MDA-MB-231 cell line
Metastasis
Microscopy
Migration inhibition
MTT assay
Protein expression level
Transwell chamber assay
Western blotting
Wound healing assay
Berberine derivative
Beta actin
Chemoattractant
Gelatinase A
Gelatinase B
Proteinase inhibitor
Unclassified drug
Article
Breast cancer
Cell migration
Cell migration assay
Cell survival
Cell viability
Chemoluminescence
Controlled study
Down regulation
Human
Human cell
MDA-MB-231 cell line
Metastasis
Microscopy
Migration inhibition
MTT assay
Protein expression level
Transwell chamber assay
Western blotting
Wound healing assay