Publication:
Antioxidant and anti-inflammatory effects of piperine on UV-B-irradiated human HaCaT keratinocyte cells

2
0

Issued Date

2020

Resource Type

Article

File Type

application/pdf

ISSN

243205

DOI

10.1016/j.lfs.2020.118607

Other identifier(s)

2-s2.0-85096213412

Rights

Srinakharinwirot University

Rights Holder(s)

Scopus

Bibliographic Citation

Life Sciences. Vol 263, (2020)

Suggested Citation

Jaisin Y., Ratanachamnong P., Wongsawatkul O., Watthammawut A., Malaniyom K., Natewong S. Antioxidant and anti-inflammatory effects of piperine on UV-B-irradiated human HaCaT keratinocyte cells. Life Sciences. Vol 263, (2020). doi:10.1016/j.lfs.2020.118607 Retrieved from: https://hdl.handle.net/20.500.14740/5097

Title

Antioxidant and anti-inflammatory effects of piperine on UV-B-irradiated human HaCaT keratinocyte cells

Author(s)

Jaisin Y.
 Ratanachamnong P.
 Wongsawatkul O.
 Watthammawut A.
 Malaniyom K.
 Natewong S.

Abstract

The increase in intracellular reactive oxygen and nitrogen species plays a key role in ultraviolet B (UV-B)-induced inflammatory responses in the human skin. Piperine exhibits many pharmacological benefits. In the present study, the photoprotective effects and the possible underlying mechanisms of the anti-inflammatory effects of piperine on UV-B-irradiated keratinocytes were investigated. Piperine exerted strong, direct scavenging effects on DPPH radicals and exhibited free radical scavenging capabilities as demonstrated by the DCFH-DA and Griess assays. Consistent with these results, 10, 20, and 40 μM piperine pretreatments attenuated UV-B irradiation-induced keratinocyte cytotoxicity as reported by the resazurin assay. The highest concentration of piperine inhibited UV-B irradiation-induced cell apoptosis, as revealed by Hoechst 33342 staining. Moreover, we demonstrated the anti-inflammatory effects of piperine using western blot analysis, real-time PCR, and ELISA. Pretreatment with piperine suppressed the activation of phosphorylated p38, JNK, and AP-1 as well as the levels of COX-2/PGE2 and iNOS synthesis, while UV-B-irradiated cells triggered the induction of these signaling molecules. These results indicated that the inhibition of these inflammatory signaling pathways might play a key role in the regulation of the anti-inflammatory effects of piperine. In addition, piperine showed stronger anti-inflammatory effects than celecoxib which served as a positive control at the same concentration. All these results suggested that the anti-inflammatory properties of piperine protected keratinocytes from UV-B-induced damage, which might be due to its antioxidant properties. Therefore, piperine may be an effective therapeutic candidate compound for the treatment of UV irradiation-induced skin inflammation. © 2020 Elsevier Inc.

Subject(s)

Celecoxib
 Cyclooxygenase 2
 Inducible nitric oxide synthase
 Interleukin 6
 Interleukin 8
 Mitogen activated protein kinase p38
 Phosphoprotein
 Piperine
 Prostaglandin E2
 Stress activated protein kinase
 Transcription factor AP 1
 1,3 benzodioxole derivative
 Alkaloid
 Amide
 Antiinflammatory agent
 Antioxidant
 Celecoxib
 Piperidine derivative
 Piperine
 Antiinflammatory activity
 Antioxidant activity
 Apoptosis
 Article
 Cell protection
 Controlled study
 Cytotoxicity
 Dermatitis
 Enzyme linked immunosorbent assay
 Enzyme synthesis
 HaCat cell line
 Human
 Human cell
 Protein expression
 Real time polymerase chain reaction
 Signal transduction
 Ultraviolet B radiation
 Western blotting
 Adverse event
 Cell line
 Comparative study
 Dose response
 Drug effect
 Inflammation
 Keratinocyte
 Pathology
 Skin
 Ultraviolet radiation
 Alkaloids
 Anti-Inflammatory Agents
 Antioxidants
 Apoptosis
 Benzodioxoles
 Celecoxib
 Cell Line
 Dose-Response Relationship, Drug
 Humans
 Inflammation
 Keratinocytes
 Piperidines
 Polyunsaturated Alkamides
 Skin
 Ultraviolet Rays

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URI

https://hdl.handle.net/20.500.14740/5097

Collections

Scopus: Year 1983-2021

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