Publication: Detection of hepatopancreatic parvovirus (HPV) infection in Penaeus monodon using PCR-ELISA
2
0
Issued Date
2002
Resource Type
File Type
application/pdf
ISSN
8908508
Other identifier(s)
2-s2.0-0036917189
Rights Holder(s)
Scopus
Bibliographic Citation
Molecular and Cellular Probes. Vol 16, No.6 (2002), p.409-413
Suggested Citation
Sukhumsirichart W., Kiatpathomchai W., Wongteerasupaya C., Withyachumnarnkul B., Flegel T.W., Boonseang V., Panyim S. Detection of hepatopancreatic parvovirus (HPV) infection in Penaeus monodon using PCR-ELISA. Molecular and Cellular Probes. Vol 16, No.6 (2002), p.409-413. doi:10.1006/mcpr.2002.0435 Retrieved from: https://hdl.handle.net/20.500.14740/6811
Abstract
A rapid and sensitive PCR-ELISA has been developed for detection of hepatopancreatic parvovirus (HPV) in Penaeus monodon. The specific primer set amplified 156 bp fragment and could detect as a little as 0.01 fg of purified HPV DNA which equivalent to three viral particles. No cross-reactivity was observed when nucleic acid templates from white spot syndromevirus, yellow-head virus, monodon baculovirus and shrimp were tested. The crude DNA simple prepared from hepatopancreas can be used as DNA template and provide a favorable result. Using this technique for detection of HPV infection in 87 carrier shrimps revealed the higher sensitivity and efficiency of detection when compared to histological examination and conventional PCR. Sixty-two percent infection was detected by PCR-ELISA from samples with HPV negative diagnosed by histological examination. Therefore, this sensitive and specific method is promisingly useful for early detection of HPV infection in broodstock, carriers and for ex situ application where large numbers of samples can be analyzed simultaneously. © 2002 Published by Elsevier Science Ltd.
Subject(s)
Animal tissue
Article
Controlled study
Cross reaction
Diagnostic accuracy
DNA determination
Early diagnosis
Enzyme linked immunosorbent assay
Gene amplification
Hepatopancreas
Histopathology
Nonhuman
Parvovirus
Polymerase chain reaction
Priority journal
Sensitivity and specificity
Shrimp
Virus detection
Virus infection
Decapoda (Crustacea)
Hepatopancreatic parvovirus of penaeid shrimp
Monodon
Monodon baculovirus
Parvovirus
Penaeus monodon
Unidentified baculovirus
Yellow head virus
Article
Controlled study
Cross reaction
Diagnostic accuracy
DNA determination
Early diagnosis
Enzyme linked immunosorbent assay
Gene amplification
Hepatopancreas
Histopathology
Nonhuman
Parvovirus
Polymerase chain reaction
Priority journal
Sensitivity and specificity
Shrimp
Virus detection
Virus infection
Decapoda (Crustacea)
Hepatopancreatic parvovirus of penaeid shrimp
Monodon
Monodon baculovirus
Parvovirus
Penaeus monodon
Unidentified baculovirus
Yellow head virus