Publication: Single-step electropolymerization on a printed sensor towards a conductive thin film polymer for the simultaneous determination of drug metabolites: 5-aminosalicylic acid and sulfapyridine
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Issued Date
2023
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File Type
application/pdf
Rights Holder(s)
Scopus
Bibliographic Citation
Analyst. Vol 148, No.13 (2023), p.3107-3116
Suggested Citation
Thangphatthanarungruang J., Chotsuwan C., Chailapakul O., Siangproh W. Single-step electropolymerization on a printed sensor towards a conductive thin film polymer for the simultaneous determination of drug metabolites: 5-aminosalicylic acid and sulfapyridine. Analyst. Vol 148, No.13 (2023), p.3107-3116. doi:10.1039/d3an00612c Retrieved from: https://hdl.handle.net/20.500.14740/12458
Other Contributor(s)
Abstract
Amino acid conductive polymers can easily form a thin film on a sensor surface by an electrochemical process. Therefore, we are pioneers in reporting the electropolymerization of l-methionine on the surface of a screen-printed graphene electrode to obtain a disposable electrochemical sensor for determining drug metabolites (5-aminosalicylic acid (5-ASA) and sulfapyridine (SPD)) of sulfasalazine (SSZ) simultaneously. In this work, the developed sensor was facilely created through a single step of electropolymerization under mild conditions (0.1 M phosphate buffer pH 7.0) using cyclic voltammetry. Important parameters in the synthesis process were systematically investigated followed by surface composition and morphology studies. Then, analytical performances, comprising sensitivity, selectivity, stability, reproducibility, and sample preparation, were carefully evaluated. Under optimal conditions, the proposed methodology demonstrated a highly sensitive and selective simultaneous detection of 5-ASA and SPD with wide linear dynamic ranges of 1-50 μM and 80-250 μM and low detection limits of 0.60 and 0.57 μM for 5-ASA and SPD, respectively. To evaluate the potential of the designed sensor, it was successfully applied by simultaneously determining 5-ASA and SPD in real human urine samples on the same day (intra-day study) and on three different days (inter-day study). © 2023 The Royal Society of Chemistry.