Publication:
Evaluation of biomimetic scaffold of gelatin-hydroxyapatite crosslink as a novel scaffold for tissue engineering: Biocompatibility evaluation with human PDL fibroblasts, human mesenchymal stromal cells, and primary bone cells

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Issued Date

2012

Resource Type

Article

File Type

application/pdf

ISSN

8853282

DOI

10.1177/0885328210391920

Other identifier(s)

2-s2.0-84863653015

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Journal of Biomaterials Applications. Vol 27, No.1 (2012), p.47-54

Suggested Citation

Rungsiyanont S., Dhanesuan N., Swasdison S., Kasugai S. Evaluation of biomimetic scaffold of gelatin-hydroxyapatite crosslink as a novel scaffold for tissue engineering: Biocompatibility evaluation with human PDL fibroblasts, human mesenchymal stromal cells, and primary bone cells. Journal of Biomaterials Applications. Vol 27, No.1 (2012), p.47-54. doi:10.1177/0885328210391920 Retrieved from: https://hdl.handle.net/20.500.14740/7016

Title

Evaluation of biomimetic scaffold of gelatin-hydroxyapatite crosslink as a novel scaffold for tissue engineering: Biocompatibility evaluation with human PDL fibroblasts, human mesenchymal stromal cells, and primary bone cells

Author(s)

Rungsiyanont S.
 Dhanesuan N.
 Swasdison S.
 Kasugai S.

Abstract

Biomimetic gelatin (gel)-hydroxyapatite (HA) composites have been prepared for studying hard tissue engineering scaffolds. However, the biocompatibility test of this form of material using these three cell types, which are periodontal ligament (PDL) fibroblast cells, human mesenchymal stromal cells (HMSc) and primary cells from human hip bone (HBc) has never been evaluated. The objective of this article is to prepare and evaluate the biocompatibility of gel-HA crosslinked scaffold for tissue engineering. Two different scaffolds were prepared: preparation (1), 2.5% gel/2.5% HA; preparation (2), 2.5% gel/5% HA. Three cell types including PDL, HMSc, and HBc were used. Assessment of biocompatibility and osteoblastic cellular responses was evaluated using a three-dimensional cell culture method and scanning electron microscopy (SEM). From SEM, it was observed that scaffold (1) exhibits stable porous formation with well-blended and dispersed HA powder. All three cell types were able to proliferate in both scaffolds. The HMSc and HBc got attached to the scaffolds to a significantly higher degree and subsequently proliferated more than PDL. The alkaline phosphatase (ALP) activities of HMSc and HBc were stronger when cultured in scaffold (S1) than (S2). It was seen that the two scaffold preparations show good biocompatibility with all three cell types tested. The better cellular responses with scaffold (S1) than (S2) might be due to the different structural and morphological characteristics, that is, scaffold (S1) retained more small-sized apatite crystals and a better developed pore configuration than scaffold (S2). Based on these findings, the biomimetically synthesized composite scaffolds have the potential to be used in hard tissue regeneration and tissue engineering fields. © The Author(s) 2010 Reprints and permissions.

Subject(s)

Alkaline phosphatase activity
 Apatite crystals
 Biomimetic scaffolds
 Bone cells
 Cell types
 Cellular response
 Composite scaffolds
 Crosslinked
 Crosslinks
 Fibroblast cells
 Gelatin-hydroxyapatite
 HA powders
 Hard tissues
 Mesenchymal stromal cells
 Morphological characteristic
 Periodontal ligament
 Pore configuration
 Primary cells
 Three dimensional cell culture
 Biocompatibility
 Biomimetics
 Cell culture
 Fibroblasts
 Hydroxyapatite
 Phosphatases
 Phosphate minerals
 Scanning electron microscopy
 Tissue
 Tissue engineering
 Scaffolds (biology)
 Gelatin
 Hydroxyapatite
 Tissue scaffold
 Article
 Biomimetics
 Cell adhesion
 Cell culture
 Cell proliferation
 Chemistry
 Cytology
 Evaluation
 Fibroblast
 Human
 Mesenchymal stroma cell
 Osteoblast
 Periodontal ligament
 Scanning electron microscopy
 Tissue engineering
 Biomimetics
 Cell Adhesion
 Cell Proliferation
 Cells, Cultured
 Durapatite
 Fibroblasts
 Gelatin
 Humans
 Mesenchymal Stromal Cells
 Microscopy, Electron, Scanning
 Osteoblasts
 Periodontal Ligament
 Tissue Engineering
 Tissue Scaffolds

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URI

https://hdl.handle.net/20.500.14740/7016

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Scopus: Year 1983-2021

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