Publication:
Simultaneous and rapid detection of white spot syndrome virus and yellow head virus infection in shrimp with a dual immunochromatographic strip test

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Issued Date

2011

Resource Type

Article

File Type

application/pdf

ISSN

1660934

DOI

10.1016/j.jviromet.2011.01.011

Other identifier(s)

2-s2.0-79952183202

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of Virological Methods. Vol 173, No.1 (2011), p.85-91

Suggested Citation

Sithigorngul P., Rukpratanporn S., Chaivisuthangkura P., Sridulyakul P., Longyant S. Simultaneous and rapid detection of white spot syndrome virus and yellow head virus infection in shrimp with a dual immunochromatographic strip test. Journal of Virological Methods. Vol 173, No.1 (2011), p.85-91. doi:10.1016/j.jviromet.2011.01.011 Retrieved from: https://hdl.handle.net/20.500.14740/7349

Title

Simultaneous and rapid detection of white spot syndrome virus and yellow head virus infection in shrimp with a dual immunochromatographic strip test

Author(s)

Sithigorngul P.
 Rukpratanporn S.
 Chaivisuthangkura P.
 Sridulyakul P.
 Longyant S.

Abstract

A strip test for the dual detection of white spot syndrome virus (WSSV) and yellow head virus (YHV) was developed using monoclonal antibodies (MAbs) specific to the WSSV major envelope protein VP28 (W1 and W30) and the YHV nucleocapsid protein p20 (Y19 and Y21). The MAbs W30 and Y19 were conjugated with colloidal gold and sprayed onto a glass fiber pad that was placed adjacent to a sample chamber. The MAbs W1 and Y21 and the goat anti-mouse immunoglobulin G (GAM) antibody were sprayed onto a nitrocellulose membrane in strips at positions designated W, Y and C, respectively. These test strips were placed in plastic cases and stored desiccated in a plastic bag. The test strips were assessed for their ability to detect WSSV and YHV simultaneously using pleopods sampled from shrimp. A pleopod homogenate in application buffer 100μl was applied to the sample chamber to flow through the nitrocellulose membrane strip, and antibody-protein complexes could be observed within 15. min. In sample from shrimp infected with WSSV and/or YHV, viral protein bound to the colloidal gold-conjugated MAbs. These complexes were captured by the MAbs at the W and/or Y test lines, resulting in the appearance of reddish-purple coloured bands. Any unbound colloidal gold-conjugated MAbs migrated pass the W and Y lines would be captured by the GAM antibody, forming a band at position C. When samples not containing WSSV and YHV proteins or containing viral proteins at below the detection limit of the test, only the band at position C was observed. The sensitivity of the test was comparable to dot blot tests using single MAbs, and ~500-fold less sensitive than a 1-step PCR test for WSSV and 1000-fold less sensitive than an RT-PCR test for YHV. Despite this lower sensitivity, the dual strip test has advantages in speed and simplicity in not requiring sophisticated equipment or specialized skills. The ability to co-detect WSSV and YHV provides simultaneously cost savings. © 2011 Elsevier B.V.

Subject(s)

Monoclonal antibody
 Virus protein
 Animal experiment
 Article
 Controlled study
 Diagnostic test accuracy study
 Ichthyophthiriasis
 Immunoassay
 Immunochromatographic strip test
 Intermethod comparison
 Nonhuman
 Priority journal
 Reverse transcription polymerase chain reaction
 RNA virus
 RNA virus infection
 Sensitivity and specificity
 Shrimp
 Test strip
 Virus detection
 White spot syndrome virus
 Yellow head virus
 Yellow head virus infection
 Animals
 Antibodies, Monoclonal
 Antibodies, Viral
 Immunoassay
 Penaeidae
 Roniviridae
 Sensitivity and Specificity
 Virology
 White spot syndrome virus 1
 Capra hircus
 Decapoda (Crustacea)
 Litopenaeus vannamei
 Shrimp white spot syndrome virus
 Yellow head virus

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URI

https://hdl.handle.net/20.500.14740/7349

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Scopus: Year 1983-2021

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