Publication:
A novel paper-based device coupled with a silver nanoparticle-modified boron-doped diamond electrode for cholesterol detection

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Issued Date

2015

Resource Type

Article

File Type

application/pdf

ISSN

32670

DOI

10.1016/j.aca.2015.08.007

Other identifier(s)

2-s2.0-84941874160

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Analytica Chimica Acta. Vol 891, (2015), p.136-143

Suggested Citation

Nantaphol S., Chailapakul O., Siangproh W. A novel paper-based device coupled with a silver nanoparticle-modified boron-doped diamond electrode for cholesterol detection. Analytica Chimica Acta. Vol 891, (2015), p.136-143. doi:10.1016/j.aca.2015.08.007 Retrieved from: https://hdl.handle.net/20.500.14740/6100

Title

A novel paper-based device coupled with a silver nanoparticle-modified boron-doped diamond electrode for cholesterol detection

Author(s)

Nantaphol S.
 Chailapakul O.
 Siangproh W.

Abstract

A novel paper-based analytical device (PAD) coupled with a silver nanoparticle-modified boron-doped diamond (AgNP/BDD) electrode was first developed as a cholesterol sensor. The AgNP/BDD electrode was used as working electrode after modification by AgNPs using an electrodeposition method. Wax printing was used to define the hydrophilic and hydrophobic areas on filter paper, and then counter and reference electrodes were fabricated on the hydrophilic area by screen-printing in house. For the amperometric detection, cholesterol and cholesterol oxidase (ChOx) were directly drop-cast onto the hydrophilic area, and H2O2 produced from the enzymatic reaction was monitored. The fabricated device demonstrated a good linearity (0.39 mg dL-1 to 270.69 mg dL-1), low detection limit (0.25 mg dL-1), and high sensitivity (49.61 μA mM-1 cm-2). The precision value for ten replicates was 3.76% RSD for 1 mM H2O2. In addition, this biosensor exhibited very high selectivity for cholesterol detection and excellent recoveries for bovine serum analysis (in the range of 99.6-100.8%). The results showed that this new sensing platform will be an alternative tool for cholesterol detection in routine diagnosis and offers the advantages of low sample/reagent consumption, low cost, portability, and short analysis time. © 2015 Elsevier B.V.

Subject(s)

Analytic equipment
 Chemical detection
 Cholesterol
 Diamonds
 Electrodes
 Hydrophilicity
 Metal nanoparticles
 Nanoparticles
 Paper
 Screen printing
 Silver
 Amperometric detection
 Boron doped diamond electrodes
 ELectrochemical detection
 Electrodeposition methods
 Hydrophilic and hydrophobic
 Paper based devices
 Paper-based analytical devices
 Silver nanoparticles
 Electrochemical electrodes
 Boron
 Cholesterol
 Cholesterol oxidase
 Diamond
 Hydrogen peroxide
 Silver nanoparticle
 Boron
 Cholesterol
 Diamond
 Metal nanoparticle
 Silver
 Adult
 Amperometry
 Analytical equipment
 Article
 Biosensor
 Bovine
 Chemical modification
 Chemical phenomena
 Cholesterol blood level
 Controlled study
 Cost control
 Electrochemical detection
 Electrode
 Electrodeposition
 Filter paper
 Hydrophilicity
 Hydrophobicity
 Limit of detection
 Lipid analysis
 Measurement precision
 Nanofabrication
 Nonhuman
 Paper based analytical device
 Portable equipment
 Printing
 Priority journal
 Reaction time
 Screen printing
 Sensitivity analysis
 Animal
 Blood
 Chemistry
 Devices
 Electrochemical analysis
 Electrode
 Equipment design
 Evaluation study
 Genetic procedures
 Paper
 Animals
 Biosensing Techniques
 Boron
 Cattle
 Cholesterol
 Diamond
 Electrochemical Techniques
 Electrodes
 Equipment Design
 Limit of Detection
 Metal Nanoparticles
 Paper
 Silver

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URI

https://hdl.handle.net/20.500.14740/6100

Collections

Scopus: Year 1983-2021

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