Publication:
Development of cross-priming amplification (CPA) combined with colorimetric and lateral flow dipstick visualization for scale drop disease virus (SDDV) detection

1
0

Issued Date

2021

Resource Type

Article

Language

eng

File Type

application/pdf

ISSN

1407775

DOI

10.1111/jfd.13448

Other identifier(s)

2-s2.0-85106619242

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of Fish Diseases. Vol 44, No.9 (2021), p.1411-1422

Suggested Citation

Prasitporn T., Senapin S., Vaniksampanna A., Longyant S., Chaivisuthangkura P. Development of cross-priming amplification (CPA) combined with colorimetric and lateral flow dipstick visualization for scale drop disease virus (SDDV) detection. Journal of Fish Diseases. Vol 44, No.9 (2021), p.1411-1422. doi:10.1111/jfd.13448 Retrieved from: https://hdl.handle.net/20.500.14740/4174

Title

Development of cross-priming amplification (CPA) combined with colorimetric and lateral flow dipstick visualization for scale drop disease virus (SDDV) detection

Author(s)

Prasitporn T.
 Senapin S.
 Vaniksampanna A.
 Longyant S.
 Chaivisuthangkura P.

Abstract

Scale drop disease virus (SDDV) is one of the most important pathogens that causes scale drop disease (SDD) in Asian sea bass (Lates calcarifer). The outbreaks of this disease are one of the factors causing substantial losses in Asian sea bass aquaculture. In this study, the uracil-DNA glycosylase (UDG)-supplemented cross-priming amplification (UCPA) combined with a colorimetric detection method using the hydroxynaphthol blue (HNB) and lateral flow dipstick (LFD) for detection of SDDV was developed. The UDG was utilized to prevent carryover contamination, and the CPA reactions can be readily observed by HNB and LFD. The CPA primers and probe were designed to target the major capsid protein (MCP) gene of the SDDV. The optimized UCPA conditions were performed at the temperature of 61°C for 60 min. The UCPA assays demonstrated specificity to SDDV without cross-reaction to other tested viruses including red-spotted grouper nervous necrosis virus (RGNNV), infectious spleen and kidney necrosis virus (ISKNV) and Lates calcarifer herpes virus (LCHV), and other bacterial species commonly found in aquatic animals. The sensitivity of the UCPA-HNB and UCPA-LFD was 100 viral copies/µl and 10 pg of extracted total DNA, which was 10-fold more sensitive than that of conventional PCR. The UCPA-HNB and UCPA-LFD assays could be used to detect the SDDV infection in all 25 confirmed SDDV-infected fish samples. Therefore, the UCPA coupled with HNB and LFD was rapid, simple and effective and might be applied for diagnosis of SDDV infection. © 2021 John Wiley & Sons Ltd

Subject(s)

Capsid protein
 Plasmid DNA
 Uracil DNA glycosidase
 Naphthalenesulfonic acid derivative
 Trisodium 3-hydroxy-4-((2Z)-2-(2-oxo-4-sulfonatonaphthalen-1-ylidene)hydrazinyl)naphthalene-2,7-disulfonate
 Amplicon
 Article
 Betanodavirus
 Colorimetry
 Controlled study
 Cross priming amplification
 Diagnostic accuracy
 DNA virus infection
 Infectious spleen and kidney necrosis virus
 Lateral flow dipstick
 Lates calcarifer
 Limit of detection
 Megalocytivirus
 Nonhuman
 Reaction temperature
 Reaction time
 Red spotted grouper nervous necrosis virus
 Scale drop disease virus
 Sensitivity and specificity
 Virus detection
 Animal
 Colorimetry
 Cross presentation
 DNA virus infection
 Fish disease
 Iridoviridae
 Isolation and purification
 Nucleic acid amplification
 Procedures
 Serology
 Veterinary medicine
 Virology
 Animals
 Colorimetry
 Cross-Priming
 DNA Virus Infections
 Fish Diseases
 Iridoviridae
 Naphthalenesulfonates
 Nucleic Acid Amplification Techniques
 Serologic Tests

View online Resources

Research Projects

Organizational Units

Journal Issue

URI

https://hdl.handle.net/20.500.14740/4174

Collections

Scopus: Year 1983-2021

Endorsement

Review

Supplemented By

Referenced By