Publication: Immunolocalization of allatostatin-like neuropeptides and their putative receptor in eyestalks of the tiger prawn, Penaeus monodon
2
0
Issued Date
2003
Resource Type
File Type
application/pdf
ISSN
1969781
Other identifier(s)
2-s2.0-0347360136
Rights Holder(s)
Scopus
Bibliographic Citation
Peptides. Vol 24, No.10 (2003), p.1563-1570
Suggested Citation
Panchan N., Bendena W.G., Bowser P., Lungchukiet P., Tobe S.S., Sithigorngul W., Chaivisuthangkura P., Rangsiruji A., Petsom A., Pewnim T., Sithigorngul P. Immunolocalization of allatostatin-like neuropeptides and their putative receptor in eyestalks of the tiger prawn, Penaeus monodon. Peptides. Vol 24, No.10 (2003), p.1563-1570. doi:10.1016/j.peptides.2003.08.015 Retrieved from: https://hdl.handle.net/20.500.14740/6629
Abstract
Allatostatin (AST)-like immunoreactivity (IR) was localized in the eyestalk of Penaeus monodon by immunohistochemistry using four anti-AST antibodies. Depending on the antisera, AST-like immunoreactivity was detected in neuronal bodies of the lamina ganglionalis, cell bodies anterior to the medulla externa and cell bodies on the anterior and posterior of the medulla terminalis. Neuronal processes in neuropiles of the medulla externa, medulla terminalis, sinus gland and nerve fibers in the optic nerve were also recognized. No IR in cell bodies or in nerve fibers was found in the medulla interna. Strong AST-like immunoreactivity was found in hundreds of cells of the X organ. The localization of AST-like peptides suggests that they function as neurotransmitters and/or neuromodulators. Antiserum to the Drosophila AST receptor (Dar-2) recognized a single protein in P. monodon eyestalk protein extracts that was identical in size to that found in Drosophila protein extracts. Using this antiserum the putative P. monodon AST receptor was localized to the sinus gland in both juvenile and adult eyestalks. To our knowledge this is the first demonstration of a neuropeptide receptor localized to the crustacean sinus gland. This suggests that ASTs may function directly on the sinus gland as a neuromodulator. In juvenile eyestalks, the putative AST receptor was also localized to neuronal X organ cells of the medulla terminalis in males but not in females. The significance of this sex-specific receptor localization is unclear but emphasizes that ASTs function within the nervous system of the eyestalk. © 2003 Elsevier Inc. All rights reserved.
Subject(s)
Allatostatin
Neuropeptide
Animal cell
Animal tissue
Conference paper
Eye
Ganglion
Immunolocalization
Nerve fiber
Nonhuman
Optic nerve
Penaeus monodon
Priority journal
Protein localization
Sex difference
Shrimp
Statistical significance
Crustacea
Decapoda (Crustacea)
Insecta
Monodon
Panthera tigris
Penaeus monodon
Terminalis
Neuropeptide
Animal cell
Animal tissue
Conference paper
Eye
Ganglion
Immunolocalization
Nerve fiber
Nonhuman
Optic nerve
Penaeus monodon
Priority journal
Protein localization
Sex difference
Shrimp
Statistical significance
Crustacea
Decapoda (Crustacea)
Insecta
Monodon
Panthera tigris
Penaeus monodon
Terminalis