Publication:
Growth inhibition and chemosensitization of human carcinoma cells by human serum albumin-coated liposomal antisense oligodeoxyribonucleotide against bcl-2

0
0

Issued Date

2012

Resource Type

Article

File Type

application/pdf

ISSN

10717544

DOI

10.3109/10717544.2012.714810

Other identifier(s)

2-s2.0-84866256041

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Drug Delivery. Vol 19, No.6 (2012), p.292-297

Suggested Citation

Weecharangsan W., Lee R.J. Growth inhibition and chemosensitization of human carcinoma cells by human serum albumin-coated liposomal antisense oligodeoxyribonucleotide against bcl-2. Drug Delivery. Vol 19, No.6 (2012), p.292-297. doi:10.3109/10717544.2012.714810 Retrieved from: https://hdl.handle.net/20.500.14740/6989

Title

Growth inhibition and chemosensitization of human carcinoma cells by human serum albumin-coated liposomal antisense oligodeoxyribonucleotide against bcl-2

Author(s)

Weecharangsan W.
 Lee R.J.

Abstract

Previous study has shown human serum albumin (HSA) coated liposomes can deliver bcl-2 antisense oligodeoxyribonucleotide (ODN) into KB carcinoma cells, and decrease bcl-2 mRNA and protein expression level. In the current study, cell growth inhibition and chemosensitization of KB cells were evaluated. Liposomes composed of dimethyldioctadecyl ammonium bromide/egg phosphatidylcholine/ α-tocopheryl polyethylene glycol 1000 succinate (58:40:2 molar ratio) complexed with bcl-2 antisense ODN and coated with HSA were examined for cell growth inhibition and sensitization to a commonly used chemotherapeutic drug, doxorubicin. HSA-coated liposomeODN complexes effectively inhibited cell growth in the range of ODN concentration of 0.457.2 M. Upon posttreatment with doxorubicin, the cytotoxicity was further significantly increased compared to the ODN complexes alone. The cytotoxicity was dependent on antisense ODN concentration, incubation time and doxorubicin concentration, and relatively independent on HSA concentration. This study suggests that HSA-coated liposomes are effective delivery vehicles for antisense ODN with potential therapeutic application and can be effectively combined with doxorubicin. © 2012 Informa Healthcare USA, Inc.

Subject(s)

Alpha tocopheryl polyethylene glycol 1000 succinate
 Antisense oligodeoxynucleotide
 Dimethyldioctadecyl ammonium bromide
 Doxorubicin
 Egg phosphatidylcholine
 Human serum albumin
 Liposome
 Messenger RNA
 Protein bcl 2
 Unclassified drug
 Article
 Carcinoma cell
 Cell growth
 Cell strain KB
 Chemosensitization
 Concentration response
 Controlled study
 Cytotoxicity
 Drug coating
 Drug delivery system
 Genetic transfection
 Growth inhibition
 Human
 Human cell
 Incubation time
 Mouth carcinoma
 Priority journal
 Protein expression
 Antibiotics, Antineoplastic
 Cell Growth Processes
 Cell Line, Tumor
 Dose-Response Relationship, Drug
 Doxorubicin
 Gene Expression Regulation, Neoplastic
 Humans
 KB Cells
 Lipids
 Liposomes
 Mouth Neoplasms
 Oligodeoxyribonucleotides, Antisense
 Proto-Oncogene Proteins c-bcl-2
 RNA, Messenger
 Serum Albumin
 Time Factors

View online Resources

Research Projects

Organizational Units

Journal Issue

URI

https://hdl.handle.net/20.500.14740/6989

Collections

Scopus: Year 1983-2021

Endorsement

Review

Supplemented By

Referenced By