Publication:
Soy isoflavones enhance β-defensin synthesis and secretion in endometrial epithelial cells with exposure to TLR3 agonist polyinosinic-polycytidylic acid

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Issued Date

2017

Resource Type

Article

File Type

application/pdf

ISSN

10467408

DOI

10.1111/aji.12694

Other identifier(s)

2-s2.0-85018643426

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

American Journal of Reproductive Immunology. Vol 78, No.4 (2017)

Suggested Citation

Srisomboon Y., Poonyachoti S., Deachapunya C. Soy isoflavones enhance β-defensin synthesis and secretion in endometrial epithelial cells with exposure to TLR3 agonist polyinosinic-polycytidylic acid. American Journal of Reproductive Immunology. Vol 78, No.4 (2017). doi:10.1111/aji.12694 Retrieved from: https://hdl.handle.net/20.500.14740/4787

Title

Soy isoflavones enhance β-defensin synthesis and secretion in endometrial epithelial cells with exposure to TLR3 agonist polyinosinic-polycytidylic acid

Author(s)

Srisomboon Y.
 Poonyachoti S.
 Deachapunya C.

Abstract

Problem: β-defensins are important innate chemical barriers that protect the endometrium from pathogen invasion. The effects of soy isoflavones, genistein and daidzein, on the expression and secretion of porcine β-defensins (PBD) in endometrial epithelial cells were investigated under normal or poly I:C-stimulated conditions. Method of study: Primary cultured porcine endometrial epithelial (PE) cells were pretreated with genistein or daidzein followed by poly I:C inoculation. During treatment, the culture media were analyzed for PBD 1-4 secretion by ELISA and the total RNA for PBD gene expression by quantitative RT-PCR. Results: Porcine endometrial epithelial cells constitutively expressed PBD 1-4 and secreted PBD-1, PBD-2, and PBD-4. Genistein and daidzein enhanced PBD-2 expression and PBD-2 and PBD-3 secretion. These compounds also potentiated PBD-2 and PBD-3 expression and secretion which were upregulated by poly I:C. Conclusion: Soy isoflavones, genistein and daidzein, could be potentially used for promoting the innate host defense of endometrium against infection. © 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd

Subject(s)

Beta defensin
 Beta defensin 1
 Beta defensin 2
 Beta defensin 3
 Beta defensin 4
 Daidzein
 Genistein
 Messenger RNA
 Polyinosinic polycytidylic acid
 Antiinfective agent
 Beta defensin
 Daidzein
 Genistein
 Isoflavone derivative
 Polyinosinic polycytidylic acid
 Animal cell
 Article
 Controlled study
 Drug effect
 Drug response
 Endometrium cell
 Enzyme linked immunosorbent assay
 Female
 Gene expression
 Inoculation
 Nonhuman
 Pig
 Primary cell culture
 Priority journal
 Protein secretion
 Protein synthesis
 Quantitative analysis
 Real time polymerase chain reaction
 Upregulation
 Animal
 Cell culture
 Cytology
 Endometrium
 Epithelium cell
 Gene expression regulation
 Genetics
 Immunology
 Innate immunity
 Metabolism
 Soybean
 Animals
 Anti-Bacterial Agents
 Beta-Defensins
 Cells, Cultured
 Endometrium
 Epithelial Cells
 Female
 Gene Expression Regulation
 Genistein
 Immunity, Innate
 Isoflavones
 Poly I-C
 Soybeans
 Swine

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URI

https://hdl.handle.net/20.500.14740/4787

Collections

Scopus: Year 1983-2021

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