Publication:
17β-Estradiol reduces nitrotyrosine immunoreactivity and increases SOD1 and SOD2 immunoreactivity in nigral neurons in male mice following MPTP insult

2
0

Issued Date

2007

Resource Type

Article

File Type

application/pdf

ISSN

68993

DOI

10.1016/j.brainres.2007.05.076

Other identifier(s)

2-s2.0-34547590252

Rights Holder(s)

Scopus

Bibliographic Citation

Brain Research. Vol 1164, No.1 (2007), p.24-31

Suggested Citation

Tripanichkul W., Sripanichkulchai K., Duce J.A., Finkelstein D.I. 17β-Estradiol reduces nitrotyrosine immunoreactivity and increases SOD1 and SOD2 immunoreactivity in nigral neurons in male mice following MPTP insult. Brain Research. Vol 1164, No.1 (2007), p.24-31. doi:10.1016/j.brainres.2007.05.076 Retrieved from: https://hdl.handle.net/20.500.14740/4370

Title

17β-Estradiol reduces nitrotyrosine immunoreactivity and increases SOD1 and SOD2 immunoreactivity in nigral neurons in male mice following MPTP insult

Author(s)

Tripanichkul W.
 Sripanichkulchai K.
 Duce J.A.
 Finkelstein D.I.

Abstract

Emerging evidence suggests the beneficial effects of estrogen on Parkinson's disease (PD), yet the mechanisms of action implicated remain elusive. While experimental evidence suggests that estrogen possesses potent antioxidative properties, it is still unknown whether the hormone exhibits a neuroprotection in a PD animal model through its antioxidant activities. This study therefore investigated the effects of 17β-estradiol (E2) on the immunoreactivity of nigral neurons and glia for nitrotyrosine (NT, a stable marker for oxidative stress), Cu/Zn superoxide dismutase (SOD1) and Mn superoxide dismutase (SOD2) in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse model. Adult male mice were treated with E2 or vehicle for 11 days during which they were injected with MPTP or saline on the sixth day. The brains were collected on day 11 and quantitative immunohistochemistry was used to assess the number of NT-, SOD1- and SOD2-immunoreactive (IR) cells in the substantia nigra pars compacta (SNpc). In saline-treated group, E2 decreased NT-IR neuronal number and raised SOD1 and SOD2 expression in neurons and glia in the SNpc. MPTP induced a significant increase in the number of NT- and SOD2-IR neurons, but decreased the number of SOD1-IR neurons. MPTP also triggered a significant increase of SOD2- and SOD1-IR glial number. E2 pretreatment in MPTP mice reduced the number of NT-IR neurons, increased the number of SOD1- and SOD2-IR neurons, but did not alter the MPTP effect on glia immunoreactive to either SOD. Stimulation of SOD1 and SOD2 expression in nigral neurons suggests that E2 provides neuroprotection against MPTP-induced oxidative stress, partly through its ability to act as an antioxidant. © 2007 Elsevier B.V. All rights reserved.

Subject(s)

1,2,3,6 tetrahydro 1 methyl 4 phenylpyridine
 3 nitrotyrosine
 Copper zinc superoxide dismutase
 Estradiol
 Manganese superoxide dismutase
 1,2,3,6 tetrahydro 1 methyl 4 phenylpyridine
 3 nitrotyrosine
 3-nitrotyrosine
 Biological marker
 Copper zinc superoxide dismutase
 Drug derivative
 Estradiol
 Manganese superoxide dismutase
 Neuroprotective agent
 Superoxide dismutase
 Tyrosine
 Unclassified drug
 Animal cell
 Animal experiment
 Article
 Cell count
 Controlled study
 Enzyme activation
 Female
 Glia cell
 Immunohistochemistry
 Immunoreactivity
 Male
 Mouse
 Nerve cell
 Neuroprotection
 Nonhuman
 Oxidative stress
 Priority journal
 Protein expression
 Substantia nigra
 Animal
 C57BL mouse
 Drug effect
 Enzymology
 Glia
 Metabolism
 Nerve cell
 Oxidative stress
 Parkinsonism
 Pathophysiology
 Physiology
 Substantia nigra
 Upregulation
 Animalia
 Mus
 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
 Animals
 Biological Markers
 Cell Count
 Estradiol
 Male
 Mice
 Mice, Inbred C57BL
 Neuroglia
 Neurons
 Neuroprotective Agents
 Oxidative Stress
 Parkinsonian Disorders
 Substantia Nigra
 Superoxide Dismutase
 Tyrosine
 Up-Regulation

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URI

https://hdl.handle.net/20.500.14740/4370

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Scopus: Year 1983-2021

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