Publication:
Association between hepatitis C virus and very-low-density lipoprotein (VLDL)/LDL analyzed in iodixanol density gradients

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Issued Date

2006

Resource Type

Article

File Type

application/pdf

ISSN

0022538X

DOI

10.1128/JVI.80.5.2418-2428.2006

Other identifier(s)

2-s2.0-33144465371

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Journal of Virology. Vol 80, No.5 (2006), p.2418-2428

Suggested Citation

Nielsen S.U., Bassendine M.F., Burt A.D., Martin C., Pumeechockchai W., Toms G.L. Association between hepatitis C virus and very-low-density lipoprotein (VLDL)/LDL analyzed in iodixanol density gradients. Journal of Virology. Vol 80, No.5 (2006), p.2418-2428. doi:10.1128/JVI.80.5.2418-2428.2006 Retrieved from: https://hdl.handle.net/20.500.14740/5923

Title

Association between hepatitis C virus and very-low-density lipoprotein (VLDL)/LDL analyzed in iodixanol density gradients

Author(s)

Nielsen S.U.
 Bassendine M.F.
 Burt A.D.
 Martin C.
 Pumeechockchai W.
 Toms G.L.

Abstract

Hepatitis C virus (HCV) RNA circulates in the blood of persistently infected patients in lipoviroparticles (LVPs), which are heterogeneous in density and associated with host lipoproteins and antibodies. The variability and lability of these virus-host complexes on fractionation has hindered our understanding of the structure of LVP and determination of the physicochemical properties of the HCV virion. In this study, HCV from an antibody-negative immunodeficient patient was analyzed using three fractionation techniques, NaBr gradients, isotonic iodixanol, and sucrose gradient centrifugation. Iodixanol gradients were shown to best preserve host lipoprotein-virus complexes, and all HCV RNA was found at densities below 1.13 g/ml, with the majority at low density, ≤1.08 g/ml. Immunoprecipitation with polyclonal antibodies against human ApoB and ApoE precipitated 91.8% and 95.0% of HCV with low density, respectively, suggesting that host lipoprotein is closely associated with HCV in a particle resembling VLDL. Immunoprecipitation with antibodies against glycoprotein E2 precipitated 25% of HCV with low density, providing evidence for the presence of E2 in LVPs. Treatment of serum with 0.5% deoxycholic acid in the absence of salt produced HCV with a density of 1.12 g/ml and a sedimentation coefficient of 215S. The diameters of these particles were calculated as 54 nm. Treatment of serum with 0.18% NP-40 produced HCV with a density of 1.18 g/ml, a sedimentation coefficient of 180S, and a diameter of 42 nm. Immunoprecipitation analysis showed that ApoB remained associated with HCV after treatment of serum with deoxycholic acid or NP-40, whereas ApoE was removed from HCV with these detergents. Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Subject(s)

Apolipoprotein B
 Apolipoprotein E
 Deoxycholic acid
 Detergent
 Iodixanol
 Lipoprotein
 Low density lipoprotein
 Polyclonal antibody
 Sodium bromide
 Very low density lipoprotein
 Virus RNA
 Article
 Clinical article
 Controlled study
 Density
 Density gradient
 Fractionation
 Hepatitis C
 Hepatitis C virus
 Human
 Human tissue
 Immune deficiency
 Immunoprecipitation
 Nonhuman
 Particle size
 Persistent infection
 Physical chemistry
 Priority journal
 Sedimentation
 Serum
 Sucrose gradient
 Virion
 Virus cell interaction
 Virus load
 Virus particle
 Apolipoproteins B
 Apolipoproteins E
 Bromides
 Centrifugation, Density Gradient
 Common Variable Immunodeficiency
 Deoxycholic Acid
 Detergents
 Hepacivirus
 Hepatitis C Antibodies
 Humans
 Immunoprecipitation
 Lipoproteins, VLDL
 Macromolecular Substances
 Polyethylene Glycols
 RNA, Viral
 Sodium Compounds
 Sucrose
 Triiodobenzoic Acids
 Viral Envelope Proteins
 Hepatitis C virus

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URI

https://hdl.handle.net/20.500.14740/5923

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Scopus: Year 1983-2021

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