Publication:
Paper-based colorimetric biosensor of blood alcohol with in-situ headspace separation of ethanol from whole blood

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Issued Date

2020

Resource Type

Article

File Type

application/pdf

ISSN

32670

DOI

10.1016/j.aca.2019.12.043

Other identifier(s)

2-s2.0-85077544266

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Analytica Chimica Acta. Vol 1103, (2020), p.115-121

Suggested Citation

Thepchuay Y., Sonsa-Ard T., Ratanawimarnwong N., Auparakkitanon S., Sitanurak J., Nacapricha D. Paper-based colorimetric biosensor of blood alcohol with in-situ headspace separation of ethanol from whole blood. Analytica Chimica Acta. Vol 1103, (2020), p.115-121. doi:10.1016/j.aca.2019.12.043 Retrieved from: https://hdl.handle.net/20.500.14740/4623

Title

Paper-based colorimetric biosensor of blood alcohol with in-situ headspace separation of ethanol from whole blood

Author(s)

Thepchuay Y.
 Sonsa-Ard T.
 Ratanawimarnwong N.
 Auparakkitanon S.
 Sitanurak J.
 Nacapricha D.

Abstract

This work presents a novel development that exploits the concept of in-situ gas-separation together with a specific enzymatic colorimetric detection to produce a portable biosensor called “Blood Alcohol Micro-pad” for direct quantitation of ethanol in whole blood. The thin square device (25 mm × 25 mm × 1.8 mm) comprises two layers of patterned filter paper held together with a double-sided mounting tape with an 8-mm circular hole (the headspace). In operation, the reagent is deposited on one layer and covered with sticky tape. Then 8 μL of a blood sample is dispensed onto the opposite layer and covered with sticky tape. Diffusion of ethanol across the 1.6 mm narrow headspace permits selective detection of ethanol by the enzymatic reagents deposited on the opposite layer. This reagent zone contains alcohol oxidase, horseradish peroxidase and 2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt, as the chromogenic reagent. The color intensity, measured from the recorded digital image, resulting from the enzymatic assay of ethanol, correlates with the concentration of blood alcohol. The results obtained with spiked mice and sheep blood samples, using an external calibration in the range of 1–120 mg dL−1ethanol, gave recoveries of 93.2–104.4% (n = 12). The “Blood Alcohol Micro-pad” gave good precision with %RSD <1 (50 mg dL−1 ethanol, n = 10) and limit of quantification (10SD of intercept/slope) of 11.56 mg dL−1. The method was successfully validated against a headspace gas chromatography-mass spectrometric method. It has good potential for development as a simple and convenient blood alcohol sensor for on-site testing. © 2019 Elsevier B.V.

Subject(s)

Analytic equipment
 Biosensors
 Color
 Colorimetry
 Ethanol
 Gas chromatography
 Mammals
 Mass spectrometry
 Separation
 ABTS
 Blood alcohols
 Colorimetric biosensors
 Headspaces
 Paper-based analytical devices
 Whole blood
 Blood
 2,2' azinobis(3 ethylbenzothiazoline 6 sulfonic acid)
 Alcohol
 Alcohol oxidase
 Horseradish peroxidase
 Reagent
 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
 Alcohol
 Alcohol dehydrogenase
 Benzothiazole derivative
 Dyes, reagents, indicators, markers and buffers
 Sulfonic acid derivative
 Accuracy
 Article
 Blood sampling
 Calibration
 Color
 Colorimetry
 Concentration (parameter)
 Diffusion
 Enzymatic assay
 Mass fragmentography
 Mouse
 Nonhuman
 Priority journal
 Reaction time
 Separation technique
 Sheep
 Validation process
 Animal
 Blood
 Chemistry
 Colorimetry
 Devices
 Enzymology
 Genetic procedures
 Horseradish
 Paper
 Procedures
 Reproducibility
 Alcohol Oxidoreductases
 Animals
 Armoracia
 Benzothiazoles
 Biosensing Techniques
 Colorimetry
 Ethanol
 Horseradish Peroxidase
 Indicators and Reagents
 Mice
 Paper
 Reproducibility of Results
 Sulfonic Acids

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URI

https://hdl.handle.net/20.500.14740/4623

Collections

Scopus: Year 1983-2021

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