Publication:
ESI-MS investigation of an equilibrium between a bimolecular quadruplex DNA and a duplex DNA/RNA hybrid

2
0

Issued Date

2015

Resource Type

Article

File Type

application/pdf

ISSN

10440305

DOI

10.1007/s13361-015-1121-2

Other identifier(s)

2-s2.0-84934931455

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Journal of the American Society for Mass Spectrometry. Vol 26, No.7 (2015), p.1165-1173

Suggested Citation

Birrento M.L., Bryan T.M., Samosorn S., Beck J.L. ESI-MS investigation of an equilibrium between a bimolecular quadruplex DNA and a duplex DNA/RNA hybrid. Journal of the American Society for Mass Spectrometry. Vol 26, No.7 (2015), p.1165-1173. doi:10.1007/s13361-015-1121-2 Retrieved from: https://hdl.handle.net/20.500.14740/6124

Title

ESI-MS investigation of an equilibrium between a bimolecular quadruplex DNA and a duplex DNA/RNA hybrid

Author(s)

Birrento M.L.
 Bryan T.M.
 Samosorn S.
 Beck J.L.

Abstract

Electrospray ionization mass spectrometry (ESI-MS) conditions were optimized for simultaneous observation of a bimolecular qDNA and a Watson-Crick base-paired duplex DNA/RNA hybrid. The DNA sequence used was telomeric DNA, and the RNA contained the template for telomerase-mediated telomeric DNA synthesis. Addition of RNA to the quadruplex DNA (qDNA) resulted in formation of the duplex DNA/RNA hybrid. Melting profiles obtained using circular dichroism spectroscopy confirmed that the DNA/RNA hybrid exhibited greater thermal stability than the bimolecular qDNA in solution. Binding of a 13-substituted berberine (1) derivative to the bimolecular qDNA stabilized its structure as evidenced by an increase in its stability in the mass spectrometer, and an increase in its circular dichroism (CD) melting temperature of 10°C. The DNA/RNA hybrid did not bind the ligand extensively and its thermal stability was unchanged in the presence of (1). The qDNA-ligand complex resisted unfolding in the presence of excess RNA, limiting the formation of the DNA/RNA hybrid. Previously, it has been proposed that DNA secondary structures, such as qDNA, may be involved in the telomerase mechanism. DNA/RNA hybrid structures occur at the active site of telomerase. The results presented in the current work show that if telomeric DNA was folded into a qDNA structure, it is possible for a DNA/RNA hybrid to form as is required during template alignment. The discrimination of ligand (1) for binding to the bimolecular qDNA over the DNA/RNA hybrid positions it as a useful compound for probing the role(s), if any, of antiparallel qDNA in the telomerase mechanism. [Figure not available: see fulltext.] © 2015 American Society for Mass Spectrometry.

Subject(s)

Bins
 Circular dichroism spectroscopy
 Complexation
 Dichroism
 DNA
 Electrodeposition
 Electrospray ionization
 Ionization
 Ligands
 Mass spectrometry
 Melting
 Oncogenic viruses
 RNA
 Spectrometers
 Thermodynamic stability
 Transcription
 DNA secondary structures
 Electrospray ionization mass spectrometry
 Hybrid structure
 Melting profiles
 Quadruplex dnas
 Simultaneous observation
 Telomerase
 Transcriptional control
 Nucleic acids
 Berberine derivative
 DNA
 Double stranded DNA
 Guanine quadruplex
 RNA
 Telomerase
 Article
 Circular dichroism
 DNA RNA hybridization
 DNA sequence
 DNA synthesis
 Electrospray mass spectrometry
 Protein secondary structure
 Telomere
 Thermostability
 Chemistry
 Electrospray mass spectrometry
 Procedures
 DNA
 G-Quadruplexes
 RNA
 Spectrometry, Mass, Electrospray Ionization
 Telomerase

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URI

https://hdl.handle.net/20.500.14740/6124

Collections

Scopus: Year 1983-2021

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