Publication:
CTLA-4 and its ligands on the surface of T- and B-lymphocyte subsets in chronic hepatitis B virus infection

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Issued Date

2013

Resource Type

Article

File Type

application/pdf

ISSN

1252208

Other identifier(s)

2-s2.0-84876849378

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of the Medical Association of Thailand. Vol 96, No.SUPPL.1 (2013), p.S54-S59

Suggested Citation

Wongjitrat C., Sukwit S., Chuenchitra T., Seangjaruk P., Rojanasang P., Romputtan P., Srisurapanon S. CTLA-4 and its ligands on the surface of T- and B-lymphocyte subsets in chronic hepatitis B virus infection. Journal of the Medical Association of Thailand. Vol 96, No.SUPPL.1 (2013), p.S54-S59. Retrieved from: https://hdl.handle.net/20.500.14740/6767

Title

CTLA-4 and its ligands on the surface of T- and B-lymphocyte subsets in chronic hepatitis B virus infection

Author(s)

Wongjitrat C.
 Sukwit S.
 Chuenchitra T.
 Seangjaruk P.
 Rojanasang P.
 Romputtan P.
 Srisurapanon S.

Abstract

Background: During chronic hepatitis B virus (CHB) infection, a number of co-stimulatory, co-inhibitory molecules and theirs ligands play a prominent role in the immune-regulation. Objective: To compare the number of peripheral-blood mononuclear cells expressing co-inhibitory marker, cytotoxic T lymphocyte associated antigen-4 (CTLA-4) and program cell death ligand-1 (PD-L1) between CHB infected patients and healthy controls. Material and Method: Peripheral-blood mononuclear cells (PBMCs) from 19 CHB-infected patients and nine healthy controls were stained with specific combinations of the following monoclonal antibodies: CD3-PE/cy5, CD4-APC, CD8-APC, CD152-PE (CTLA-4), CD19PE/Cy5, CD80-FITC (B7-1), CD86-PE (B7-2) and CD274-FITC (B7-H1) according to standard protocol. Results: The frequencies of B-lymphocyte expressing B7-1, B7-2 and B7-H1 of CHB-infected patients and healthy controls were not shown any statistical differences. The mean percentage of B-lymphocyte with B7-2 molecule was higher than those with B7-1 molecules in both infected- and non-infected groups. In contrast, the frequencies of T-lymphocyte subsets, CD3+, CD4+ and CD8+ expressing CTLA-4 molecules in CHB-infected patients were significantly higher than those in healthy controls with p = 0.04, 0.01 and 0.04 respectively. Conclusion: An increase in percentage of circulating CD4+/CD152+ (T-cell) was observed in CHB-infected patients. A small but significant increase in percentage of CD8+/CD152+ T-cells raises the possibility that CTLA-4 are involved in the development of HBV-specific CD8+ T-cell exhaustion. Overall, CD4+ and CD8+ T-cells presenting CTLA-4 might contribute to the impaired immune response and likely to be a factor influencing in failure of immunological control of the persisting pathogens.

Subject(s)

B7 antigen
 CD19 antibody
 CD3 antibody
 CD4 antibody
 CD86 antigen
 Cytotoxic T lymphocyte antigen 4 antibody
 Monoclonal antibody
 OKT 8
 Programmed death 1 ligand 1
 Adult
 Aged
 Antigen expression
 Article
 B lymphocyte
 Chronic hepatitis
 Clinical article
 Controlled study
 Female
 Flow cytometry
 Hepatitis B
 Hepatitis B virus
 Human
 Immune response
 Immunoregulation
 Lymphocyte subpopulation
 Male
 Peripheral blood mononuclear cell
 T lymphocyte
 Adult
 Aged
 Aged, 80 and over
 Antibodies, Monoclonal
 Antigens, CD274
 B-Lymphocyte Subsets
 Case-Control Studies
 CTLA-4 Antigen
 Female
 Flow Cytometry
 Hepatitis B, Chronic
 Humans
 Male
 Middle Aged
 Statistics, Nonparametric
 T-Lymphocyte Subsets

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URI

https://hdl.handle.net/20.500.14740/6767

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Scopus: Year 1983-2021

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