Publication:
Paper-based amperometric sensor for determination of acetylcholinesterase using screen-printed graphene electrode

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Issued Date

2018

Resource Type

Article

File Type

application/pdf

ISSN

399140

DOI

10.1016/j.talanta.2017.08.096

Other identifier(s)

2-s2.0-85033345539

Rights Holder(s)

Scopus

Bibliographic Citation

Talanta. Vol 178, (2018), p.1017-1023

Suggested Citation

Panraksa Y., Siangproh W., Khampieng T., Chailapakul O., Apilux A. Paper-based amperometric sensor for determination of acetylcholinesterase using screen-printed graphene electrode. Talanta. Vol 178, (2018), p.1017-1023. doi:10.1016/j.talanta.2017.08.096 Retrieved from: https://hdl.handle.net/20.500.14740/3715

Title

Paper-based amperometric sensor for determination of acetylcholinesterase using screen-printed graphene electrode

Author(s)

Panraksa Y.
 Siangproh W.
 Khampieng T.
 Chailapakul O.
 Apilux A.

Abstract

A simple paper-based sensor with electrochemical detection was first developed for rapid, selective, and sensitive determination of acetylcholinesterase (AChE). The screen-printed graphene electrode was used as working electrode providing sensitivity for the sensor. The amperometric detection of AChE is based on the determination of thiocholine (TCh) produced from hydrolysis of acetylthiocholine chloride (ATCh) by AChE. To detect TCh, the ATCh immobilized sheet was stacked onto the detection sheet using double adhesive tape, then samples of AChE were dropped on the back side of an ATCh immobilized sheet with only 1 min of incubation time. To avoid interference, glutathione (GSH), the potential of 0.5 V vs. Ag/AgCl was applied onto a graphene electrode and the current, which depends on AChE concentration, was measured. Under optimized conditions, the limit of detection (LOD) from the experiment of AChE determination was 0.1 U/mL with AChE concentration in range of 0.1–15 U/mL. The data correlated well with the data obtained using spectrophotometric method. The developed sensor had been successfully applied to detect AChE in blood samples. © 2017 Elsevier B.V.

Subject(s)

Chemical detection
 Chlorine compounds
 Electrochemical electrodes
 Graphene
 Graphite electrodes
 Spectrophotometry
 Acetylcholinesterase
 Amperometric detection
 ELectrochemical detection
 Graphene electrodes
 Optimized conditions
 Paper-based sensors
 Screen-printed graphene electrodes
 Spectro-photometric method
 Amperometric sensors
 Acetylcholinesterase
 Acetylthiocholine
 Graphite
 Thiocholine
 Animal
 Chemistry
 Devices
 Electrochemistry
 Electrode
 Electrophorus
 Enzyme assay
 Kinetics
 Metabolism
 PH
 Printing
 Acetylcholinesterase
 Acetylthiocholine
 Animals
 Electrochemistry
 Electrodes
 Electrophorus
 Enzyme Assays
 Graphite
 Hydrogen-Ion Concentration
 Kinetics
 Printing
 Thiocholine

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URI

https://hdl.handle.net/20.500.14740/3715

Collections

Scopus: Year 1983-2021

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