Publication: High Potential Decolourisation of Textile Dyes from Wastewater by Manganese Peroxidase Production of Newly Immobilised Trametes hirsuta PW17-41 and FTIR Analysis
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Issued Date
2022
Resource Type
Language
eng
File Type
application/pdf
ISSN
20762607
Rights Holder(s)
Scopus
Bibliographic Citation
ZooKeys. Vol 2022, No.1125 (2022), p.115-158
Suggested Citation
Thampraphaphon B., Phosri C., Pisutpaisal N., Thamvithayakorn P., Chotelersak K., Sarp S., Suwannasai N. High Potential Decolourisation of Textile Dyes from Wastewater by Manganese Peroxidase Production of Newly Immobilised Trametes hirsuta PW17-41 and FTIR Analysis. ZooKeys. Vol 2022, No.1125 (2022), p.115-158. doi:10.3390/microorganisms10050992 Retrieved from: https://hdl.handle.net/20.500.14740/9326
Abstract
Coloured wastewater from the textile industry is a very serious global problem. Among 16 different white-rot fungal isolates, Trametes hirsuta PW17-41 revealed high potential for decolourisation of mixed textile dyes (Navy EC-R, Ruby S3B and Super Black G) from real industrial wastewater samples. The efficiency of dye decolourisation was evaluated using the American Dye Manufacturers’ Institute (ADMI) standard methodology. The suitable support for fungal mycelium immobilisation was nylon sponges. The optimal dye decolourisation (95.39%) was achieved by using palm sugar and ammonium nitrate as carbon and nitrogen sources, respectively. The initial pH was 5 and the agitation speed was 100 rpm at 30 °C. The ADMI values of textile dyes decreased from 2475 to 114 within two days, reducing the treatment time from seven days before optimisation. The major mechanism of dye decolourisation was biodegradation, which was confirmed by UV– visible and FTIR spectra. Manganese peroxidase (MnP) (4942 U L−1) was found to be the main enzyme during the decolourisation process at an initial dye concentration of 21,200 ADMI. The results indicated the strong potential of immobilised fungal cells to remove high concentrations of textile dyes from industrial wastewater and their potential ability to produce high MnP and laccase activities that can be used in further application. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.