Publication: A fast and highly sensitive detection of cholesterol using polymer microfluidic devices and amperometric system
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Issued Date
2011
Resource Type
File Type
application/pdf
ISSN
399140
Other identifier(s)
2-s2.0-79958123313
Rights Holder(s)
Scopus
Bibliographic Citation
Talanta. Vol 84, No.5 (2011), p.1323-1328
Suggested Citation
Ruecha N., Siangproh W., Chailapakul O. A fast and highly sensitive detection of cholesterol using polymer microfluidic devices and amperometric system. Talanta. Vol 84, No.5 (2011), p.1323-1328. doi:10.1016/j.talanta.2011.02.040 Retrieved from: https://hdl.handle.net/20.500.14740/7308
Author(s)
Abstract
In this work, the rapid detection of cholesterol using poly(dimethylsiloxane) microchip capillary electrophoresis, based on the coupling of enzymatic assays and electrochemical detection, was developed. Direct amperometric detection for poly(dimethylsiloxane) (PDMS) microchip capillary electrophoresis was successfully applied to quantify cholesterol levels. Factors influencing the performance of the method (such as the concentration and pH value of buffer electrolyte, concentration of cholesterol oxidase enzyme (ChOx), effect of solvent on the cholesterol solubility, and interferences) were carefully investigated and optimized. The migration time of hydrogen peroxide, product of the reaction, was less than 100 s when using 40 mM phosphate buffer at pH 7.0 as the running buffer, a concentration of 0.68 U/mL of the ChOx, a separation voltage of +1.6 kV, an injection time of 20 s, and a detection potential of +0.5 V. PDMS microchip capillary electrophoresis showed linearity between 38.7 μg/dL (1 μM) and 270.6 mg/dL (7 mM) for the cholesterol standard; the detection limit was determined as 38.7 ng/dL (1 nM). To demonstrate the potential of this assay, the proposed method was applied to quantify cholesterol in bovine serum. The percentages of recoveries were assessed over the range of 98.9-101.8%. The sample throughput was found to be 60 samples per hour. Therefore, PDMS microchip capillary electrophoresis, based on the coupling of enzymatic assays and electrochemical detection, is very rapid, accurate and sensitive method for the determination of cholesterol levels. © 2011 Elsevier B.V. All rights reserved.
Subject(s)
Assays
Capillary electrophoresis
Cholesterol
Electrolytes
Microchannels
Microprocessor chips
Amperometric detection
ELectrochemical detection
Enzymatic assay
Highly sensitive detections
Microchip capillary electrophoresis
PDMS microchips
Polydimethylsiloxane PDMS
Polymer microfluidic devices
Chemical detection
Capillary electrophoresis
Cholesterol
Electrolytes
Microchannels
Microprocessor chips
Amperometric detection
ELectrochemical detection
Enzymatic assay
Highly sensitive detections
Microchip capillary electrophoresis
PDMS microchips
Polydimethylsiloxane PDMS
Polymer microfluidic devices
Chemical detection