Publication:
Differential effects of heat stress on fibre capillarisation in tenotomised soleus and plantaris muscles

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Issued Date

2018

Resource Type

Article

File Type

application/pdf

ISSN

2656736

DOI

10.1080/02656736.2017.1350758

Other identifier(s)

2-s2.0-85025173317

Rights Holder(s)

Scopus

Bibliographic Citation

International Journal of Hyperthermia. Vol 34, No.4 (2018), p.432-441

Suggested Citation

Hirunsai M., Srikuea R. Differential effects of heat stress on fibre capillarisation in tenotomised soleus and plantaris muscles. International Journal of Hyperthermia. Vol 34, No.4 (2018), p.432-441. doi:10.1080/02656736.2017.1350758 Retrieved from: https://hdl.handle.net/20.500.14740/4696

Title

Differential effects of heat stress on fibre capillarisation in tenotomised soleus and plantaris muscles

Author(s)

Hirunsai M.
 Srikuea R.

Abstract

Purpose: Capillary regression is commonly observed in response to disuse muscle atrophy. Heat stress is known to alleviate muscle atrophy, while effect of heat exposure on capillary adaptation following disuse atrophy is not defined. Here, we examined the effect of heat treatment on capillarisation and the associated signalling in slow-oxidative soleus and fast-glycolytic plantaris muscles following Achilles tendon ablation (tenotomy). Materials and methods: Male Wistar rats were assigned into control (CON), control with heat stress (CON + HEAT), tenotomy (TEN) and tenotomy with heat stress (TEN + HEAT) groups. Tenotomy was induced for 8 days in TEN and TEN + HEAT groups. Heat stress was maintained at 40.5–41.5 °C, 30 min for 7 days. Results: Tenotomy resulted in reduction of capillary-to-fibre ratio, decreased VEGFR-2 and increased TSP-1 in soleus muscle, whereas VEGF protein expression remained unaffected. Tenotomy had no effect on capillary distribution and angiogenic signalling in plantaris muscle. These results were concomitant with larger reduction of cross-sectional area (CSA) in MHC type I and II myofibres of soleus compared to plantaris muscles. Interestingly, heat stress increased VEGFR-2 and attenuated TSP-1 protein expression in tenotomised soleus, but not plantaris muscles. Additionally, CSA of both type I and type II myofibres was greater in tenotomised soleus than plantaris muscles after heat treatment. Conclusions: Heat stress mitigated effect of tenotomy-induced capillary regression in a fibre-type-specific response, in part, by shifting the balance between angiogenic and angiostatic regulators. These results suggest beneficial effect of heat treatment for maintaining microcirculation in disuse muscle atrophy. © 2017 Informa UK Limited, trading as Taylor & Francis Group.

Subject(s)

Myosin heavy chain
 Thrombospondin 1
 Vasculotropin receptor 2
 Kdr protein, rat
 Thrombospondin 1
 Thrombospondin 1, rat
 Vascular endothelial growth factor A, rat
 Vasculotropin A
 Vasculotropin receptor 2
 Achilles tendon
 Angiogenesis
 Animal experiment
 Animal model
 Article
 Capillary
 Clinical evaluation
 Comparative study
 Controlled study
 Glycolysis
 Heat stress
 Heat treatment
 Major histocompatibility complex
 Male
 Microcirculation
 Muscle atrophy
 Muscle fibril
 Muscle mass
 Nonhuman
 Plantaris muscle
 Protein expression
 Rat
 Soleus muscle
 Tenotomy
 Animal
 Capillary
 Heat shock response
 Metabolism
 Muscle atrophy
 Pathophysiology
 Physiology
 Skeletal muscle
 Surgery
 Tenotomy
 Wistar rat
 Achilles Tendon
 Animals
 Capillaries
 Heat-Shock Response
 Male
 Muscle, Skeletal
 Muscular Atrophy
 Rats, Wistar
 Tenotomy
 Thrombospondin 1
 Vascular Endothelial Growth Factor A
 Vascular Endothelial Growth Factor Receptor-2

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URI

https://hdl.handle.net/20.500.14740/4696

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Scopus: Year 1983-2021

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