Publication: Corymine potentiates NMDA-induced currents in Xenopus oocytes expressing NR1a/NR2B glutamate receptors
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0
Issued Date
2005
Resource Type
File Type
application/pdf
ISSN
13478613
Other identifier(s)
2-s2.0-20444399567
Rights Holder(s)
Scopus
Bibliographic Citation
Journal of Pharmacological Sciences. Vol 98, No.1 (2005), p.58-65
Suggested Citation
Leewanich P., Tohda M., Takayama H., Sophasan S., Watanabe H., Matsumoto K. Corymine potentiates NMDA-induced currents in Xenopus oocytes expressing NR1a/NR2B glutamate receptors. Journal of Pharmacological Sciences. Vol 98, No.1 (2005), p.58-65. doi:10.1254/jphs.FP0050023 Retrieved from: https://hdl.handle.net/20.500.14740/6125
Abstract
Previous studies demonstrated that corymine, an indole alkaloid isolated from the leaves of Hunter zeylanica, dose-dependently inhibited strychnine-sensitive glycine-induced currents. However, it is unclear whether this alkaloid can modulate the function of the N-methyl-D-aspartate (NMDA) receptor on which glycine acts as a co-agonist via strychnine-insensitive glycine binding sites. This study aimed to evaluate the effects of corymine on NR1a/NR2B NMDA receptors expressed in Xenopus oocytes using the two-electrode voltage clamp technique. Corymine significantly potentitated the NMDA-induced currents recorded from oocytes on days 3 and 4 after cRNA injection but it showed no effect when the current was recorded on days 5 and 6. The potentiating effect of corymine on NMDA-induced currents was induced in the presence of a low concentration of glycine (≤0.1 μM). Spermine significantly enhanced the potentiating effect of corymine observed in the oocytes on days 3 and 4, while the NMDA-receptor antagonist 2-amino-5-phosphonopentanone (AP5) and the NMDA-channel blocker 5-methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10- imine (MK-801) reversed the effect of corymine. On the other hand, the nonspecific chloride channel blocker 4,4-di-isothiocyano stilbene-2,2-disulfonoc acid (DIDS) had no effect on the corymine potentiation of NMDA currents. There was no good correlation between corymine- and spermine-induced potentiation of the NMDA-current response in Xenopus oocytes. These results suggest that corymine potentiates the NMDA-induced currents by interacting with a site different from the spermine binding site. © 2005 The Japanese Pharmacological Society.
Subject(s)
2 amino 5 phosphonovaleric acid
4,4' diisothiocyanatostilbene 2,2' disulfonic acid
Chloride channel blocking agent
Complementary RNA
Corymine
Dizocilpine
Glutamate receptor
Glycine
Indole alkaloid
N methyl dextro aspartic acid
N methyl dextro aspartic acid receptor
N methyl dextro aspartic acid receptor 1a
N methyl dextro aspartic acid receptor 2B
N methyl dextro aspartic acid receptor blocking agent
Spermine
Strychnine
Unclassified drug
Animal cell
Article
Binding site
Correlation analysis
Evaluation
Hunter zeylanica
Nonhuman
Oocyte
Plant
Plant leaf
Protein expression
Seizure
Voltage clamp
Xenopus laevis
Action Potentials
Alkaloids
Animals
Binding Sites
Dose-Response Relationship, Drug
Drug Synergism
Female
Gene Expression Regulation
N-Methylaspartate
Oocytes
Plant Extracts
Plant Leaves
Receptors, N-Methyl-D-Aspartate
Xenopus laevis
4,4' diisothiocyanatostilbene 2,2' disulfonic acid
Chloride channel blocking agent
Complementary RNA
Corymine
Dizocilpine
Glutamate receptor
Glycine
Indole alkaloid
N methyl dextro aspartic acid
N methyl dextro aspartic acid receptor
N methyl dextro aspartic acid receptor 1a
N methyl dextro aspartic acid receptor 2B
N methyl dextro aspartic acid receptor blocking agent
Spermine
Strychnine
Unclassified drug
Animal cell
Article
Binding site
Correlation analysis
Evaluation
Hunter zeylanica
Nonhuman
Oocyte
Plant
Plant leaf
Protein expression
Seizure
Voltage clamp
Xenopus laevis
Action Potentials
Alkaloids
Animals
Binding Sites
Dose-Response Relationship, Drug
Drug Synergism
Female
Gene Expression Regulation
N-Methylaspartate
Oocytes
Plant Extracts
Plant Leaves
Receptors, N-Methyl-D-Aspartate
Xenopus laevis