Publication: Application of surface plasmon resonance biosensor for the detection of Candida albicans
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Issued Date
2016
Resource Type
File Type
application/pdf
ISSN
214922
Other identifier(s)
2-s2.0-84956625420
Rights Holder(s)
Scopus
Bibliographic Citation
Japanese Journal of Applied Physics. Vol 55, No.2 (2016)
Suggested Citation
Yodmongkol S., Thaweboon S., Thaweboon B., Puttharugsa C., Sutapun B., Amarit R., Somboonkaew A., Srikhirin T. Application of surface plasmon resonance biosensor for the detection of Candida albicans. Japanese Journal of Applied Physics. Vol 55, No.2 (2016). doi:10.7567/JJAP.55.02BE03 Retrieved from: https://hdl.handle.net/20.500.14740/5717
Abstract
In this study, surface plasmon resonance imaging (SPR imaging) was developed for the detection of Candida albicans which is a causal agent of oral infection. The detection was based on the sandwich assay. The capture antibody was covalently immobilized on the mixed self assemble monolayers (SAMs). The ratio of mixed SAMs between 11-mercaptoundecanoic acid and 3-mercaptopropanol was varied to find the optimal ratio for use as a sensor surface. The results showed that the suitable surface for C. albicans detection was SAM of carboxylic (mixed SAMs 1 : 0), even though mixed SAMs 1 : 40 had a high detection signal in comparison to mixed SAMs 1 : 0, but the non-specific signal was higher. The detection limit was 107 cells/ml for direct detection, and was increased to 106 cells/ml with sandwich antibody. The use of polyclonal C. albicans antibody as capture and sandwich antibody showed good selectivity against the relevant oral bacteria including Escherichia coli, Streptococcus mutan, Staphylococcus aureus, β-streptococci, and Lactobacillus casei. SPR platform in this study could detect C. albicans from the mixed microbial suspension without requirement of skillful technician. This SPR imaging biosensor could be applied for Candida identification after cultivation. © 2016 The Japan Society of Applied Physics.
Subject(s)
Antibodies
Bacteria
Biosensors
Candida
Chemical detection
Escherichia coli
Plasmons
Signal detection
Yeast
11-mercaptoundecanoic acid
Detection limits
Direct detection
Lactobacillus casei
Self-assemble monolayers
Staphylococcus aureus
Surface plasmon resonance biosensor
Surface plasmon resonance imaging
Surface plasmon resonance
Bacteria
Biosensors
Candida
Chemical detection
Escherichia coli
Plasmons
Signal detection
Yeast
11-mercaptoundecanoic acid
Detection limits
Direct detection
Lactobacillus casei
Self-assemble monolayers
Staphylococcus aureus
Surface plasmon resonance biosensor
Surface plasmon resonance imaging
Surface plasmon resonance