Publication: β-Glucan fragmentation by microfluidization and TNF-α-immunostimulating activity of fragmented β-glucans
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Issued Date
2024-04-30
Resource Type
ISSN
24058440
Scopus ID
2-s2.0-85189892667
Journal Title
Heliyon
Volume
10
Issue
8
Rights Holder(s)
SCOPUS
Bibliographic Citation
Heliyon Vol.10 No.8 (2024)
Suggested Citation
Nanta P., Buachan P., Pinket W., Srinuanchai W., Pongwan P., Sramala I., Jarussophon S., Prathumpai W., Taweechotipatr M., Ruktanonchai U.R., Kasemwong K. β-Glucan fragmentation by microfluidization and TNF-α-immunostimulating activity of fragmented β-glucans. Heliyon Vol.10 No.8 (2024). doi:10.1016/j.heliyon.2024.e29444 Retrieved from: https://hdl.handle.net/20.500.14740/20438
Corresponding Author(s)
Other Contributor(s)
Abstract
Fragmentation of β-glucans secreted by the fungus Ophiocordyceps dipterigena BCC 2073 achieved by microfluidization was investigated. The degree of β-glucan fragmentation was evaluated based on the average number of chain scissions (α). The effects on the α value of experimental variables like solid concentration of the β-glucan suspension, interaction chamber pressure, and number of passes through the microfluidizer were examined. Kinetic studies were conducted using the relationships of the α and suspension viscosity values with the number of passes. Evidence indicated that α increases with the interaction chamber pressure and the number of passes, whereas the solid concentration shows the inverted effect. Kinetic data indicated that the fragmentation rate increases with β-glucan solid concentration and interaction chamber pressure. Furthermore, since β-glucan molecular weight is a key factor determining its biological activity, the effect of β-glucans of different molecular weights produced by fragmentation on tumor necrosis factor (TNF)-α-stimulating activity in THP-1 human macrophage cells was investigated. Evidence suggested that β-glucans have an immunostimulating effect on macrophage function, in the absence of cytotoxic effects. Indeed, β-glucans characterized by a range of molecular weights produced via microfluidization exhibited promise as immunostimulatory agents.