Publication:
Multiplex RT-PCR assay for simultaneous detection of six viruses of penaeid shrimp

0
0

Issued Date

2008

Resource Type

Article

File Type

application/pdf

ISSN

8908508

DOI

10.1016/j.mcp.2007.12.005

Other identifier(s)

2-s2.0-42649113419

Rights Holder(s)

Scopus

Bibliographic Citation

Molecular and Cellular Probes. Vol 22, No.3 (2008), p.177-183

Suggested Citation

Khawsak P., Deesukon W., Chaivisuthangkura P., Sukhumsirichart W. Multiplex RT-PCR assay for simultaneous detection of six viruses of penaeid shrimp. Molecular and Cellular Probes. Vol 22, No.3 (2008), p.177-183. doi:10.1016/j.mcp.2007.12.005 Retrieved from: https://hdl.handle.net/20.500.14740/3967

Title

Multiplex RT-PCR assay for simultaneous detection of six viruses of penaeid shrimp

Author(s)

Khawsak P.
 Deesukon W.
 Chaivisuthangkura P.
 Sukhumsirichart W.

Abstract

In the present study, multiplex reverse transcription-polymerase chain reaction (mRT-PCR) was developed for simultaneously detection of six major shrimp viruses including yellow-head virus (YHV), white spot syndrome virus (WSSV), Taura syndrome virus (TSV), hepatopancreatic parvovirus (HPV), infectious hypodermal and hematopoietic necrosis virus (IHHNV) and monodon baculovirus (MBV). The six primer sets could amplify viral nucleic acids resulting in PCR products with different sizes. They were highly specific and did not cross-hybridize with other viral or shrimp nucleic acids. The sensitivity of the multiplex RT-PCR was 0.15 pg for IHHNV, 0.15 pg for TSV, 1.00 pg for HPV, 1.5 pg for MBV, 5.00 pg for WSSV and 10.00 pg for YHV. In the field application, 42 samples including whole tissue of post-larvae and hepatopancreas of Penaeus monodon collected from ponds in the central and southern parts of Thailand during 2002-2005 were examined by multiplex RT-PCR. The results revealed that a single infection was dominant and WSSV was the highest prevalence at that time. Dual infection was found in one sample. This developed multiplex RT-PCR will be useful for simultaneous detection of six major viruses of penaeid shrimp and benefit to shrimp cultured industry. © 2008 Elsevier Ltd. All rights reserved.

Subject(s)

Aquaculture
 Article
 Cross hybridization
 Gene amplification
 Hepatopancreatic parvovirus
 Infectious hypodermal and hematopoietic necrosis virus
 Larva
 Monodon baculovirus
 Nonhuman
 Nucleic acid analysis
 Nucleotide sequence
 Oligonucleotide probe
 Penaeidae
 Prevalence
 Priority journal
 Reverse transcription polymerase chain reaction
 Shrimp
 Taura syndrome virus
 Thailand
 Virus detection
 Virus infection
 Virus strain
 White spot syndrome virus
 Yellow head virus
 Animals
 DNA Primers
 DNA Viruses
 Penaeidae
 Reverse Transcriptase Polymerase Chain Reaction
 RNA Viruses
 RNA, Ribosomal
 Sensitivity and Specificity
 Decapoda (Crustacea)
 Hepatopancreatic parvovirus of penaeid shrimp
 Human papillomavirus
 Infectious hypodermal and hematopoietic necrosis virus
 Monodon baculovirus
 Mushroom bacilliform virus
 Penaeidae
 Penaeus monodon
 Shrimp white spot syndrome virus
 Taura syndrome virus
 Yellow head virus

View online Resources

Research Projects

Organizational Units

Journal Issue

URI

https://hdl.handle.net/20.500.14740/3967

Collections

Scopus: Year 1983-2021

Endorsement

Review

Supplemented By

Referenced By