Publication: The opioid receptors in inner ear of different stages of postnatal rats
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Issued Date
2003
Resource Type
File Type
application/pdf
ISSN
3785955
Other identifier(s)
2-s2.0-0141862069
Rights Holder(s)
Scopus
Bibliographic Citation
Hearing Research. Vol 184, (2003), p.1-10
Suggested Citation
Phansuwan-Pujito P., Saleema L., Mukda S., Tongjaroenbuangam W., Jutapakdeegul N., Casalotti S.O., Forge A., Dodson H., Govitrapong P. The opioid receptors in inner ear of different stages of postnatal rats. Hearing Research. Vol 184, (2003), p.1-10. doi:10.1016/S0378-5955(03)00163-1 Retrieved from: https://hdl.handle.net/20.500.14740/6635
Abstract
There is increasing evidence that the opioid system has a role in hearing. To provide further evidence for such a role, the expression of opioid receptor mRNAs and proteins in the inner ear of rats was studied during development from birth (P0) to postnatal day 16 (P16). A semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) was employed to detect changes in the expression of delta- (DOR) kappa- (KOR) and mu- (MOR) opioid receptor mRNAs in rat cochleae at P0, P4, P8 and P16. Expression of DOR mRNA levels steadily increased from P0 to P8 with no further increases by P16. KOR mRNA was expressed at a relatively high level at P0 and P4 followed by a decrease while MOR mRNA was expressed at a low level at P0 and P4 followed by an increase by P8 and P16. Immunocytochemical labelling of inner ear sections revealed unique developmental and distribution patterns of opioid receptors. In the organ of Corti DOR immunoreactivity (DOR-IR) was detected in hair cells from P4. In contrast MOR-IR was present only in supporting cells at P0-P16. In the spiral ganglion all three receptor subtypes were expressed from P0 on nerve cell soma and qualitatively appeared to increase with age. Also DOR-IR and MOR-IR were detected at P8 and P16 in nerve fibers within the spiral ganglion. In the limbus DOR-IR was detected at P8 and P16 on cells proximal to the tectorial membrane while MOR-IR was detected more distally. In general these findings demonstrate that within the inner ear each receptor subtype follows specific temporal and spatial developmental patterns, some of which may be associated to the onset of hearing. The data provide further evidence that the opioid system may play a role in the development and functioning of the inner ear. © 2003 Elsevier B.V. All rights reserved.
Subject(s)
Delta opiate receptor
Kappa opiate receptor
Messenger RNA
Mu opiate receptor
Opiate receptor
Receptor subtype
Animal experiment
Animal tissue
Article
Cochlea
Cochlea duct
Controlled study
Corti organ
Gene expression
Hair cell
Immunocytochemistry
Immunoreactivity
Inner ear
Nerve fiber
Nonhuman
Postnatal development
Priority journal
Rat
Reverse transcription polymerase chain reaction
Spiral ganglion
Tissue section
Kappa opiate receptor
Messenger RNA
Mu opiate receptor
Opiate receptor
Receptor subtype
Animal experiment
Animal tissue
Article
Cochlea
Cochlea duct
Controlled study
Corti organ
Gene expression
Hair cell
Immunocytochemistry
Immunoreactivity
Inner ear
Nerve fiber
Nonhuman
Postnatal development
Priority journal
Rat
Reverse transcription polymerase chain reaction
Spiral ganglion
Tissue section