Publication:
Dietary L-arginine normalizes endothelin-induced vascular contractions in cholesterol-fed rabbits

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Issued Date

1998

Resource Type

Article

File Type

application/pdf

ISSN

1602446

DOI

10.1097/00005344-199808000-00019

Other identifier(s)

2-s2.0-0345487006

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Journal of Cardiovascular Pharmacology. Vol 32, No.2 (1998), p.300-307

Suggested Citation

Phivthong-Ngam L., Bode-Böger S.M., Böger R.H., Böhme M., Brandes R.P., Mügge A., Frölich J.C. Dietary L-arginine normalizes endothelin-induced vascular contractions in cholesterol-fed rabbits. Journal of Cardiovascular Pharmacology. Vol 32, No.2 (1998), p.300-307. doi:10.1097/00005344-199808000-00019 Retrieved from: https://hdl.handle.net/20.500.14740/7231

Title

Dietary L-arginine normalizes endothelin-induced vascular contractions in cholesterol-fed rabbits

Author(s)

Phivthong-Ngam L.
 Bode-Böger S.M.
 Böger R.H.
 Böhme M.
 Brandes R.P.
 Mügge A.
 Frölich J.C.

Abstract

The endothelium regulates vascular function by releasing the vasodilator autacoid nitric oxide (NO) and the vasoconstrictor peptide endothelin-1 (ET- 1). Impaired activity of NO as well as excessive activity of ET-1 have been demonstrated in hypercholesterolemia and atherosclerosis. Because dietary L- arginine can restore NO function and improve abnormal endothelium-dependent relaxation in hypercholesterolemic rabbits, we examined the effects of dietary supplementation with L-arginine in cholesterol-fed rabbits on endothelium-dependent vascular relaxation and ET-1-induced vascular contraction, as well as the systemic synthesis of ET-1. Rabbits were initially fed a diet enriched with 1% cholesterol for 4 weeks, followed by 0.5% cholesterol alone or supplemented with 2% L-arginine in drinking water during the next 12 weeks. Cholesterol feeding impaired endothelium-dependent relaxation of rabbit aortic rings ex vivo and increased urinary immunoreactive ET-1 excretion, along with decreased urinary nitrate excretion, an index of NO production. L-Arginine partially restored endothelium-dependent relaxation in parallel to increased urinary nitrate excretion and decreased urinary immunoreactive ET-1 excretion. Selective inhibition of ET-A receptors with BQ123 partially restored endothelium- dependent relaxation in hypercholesterolemic rabbits but had no effect on arterial rings from rabbits supplemented with L-arginine or from control animals. The contractile vascular response of aortic rings to exogenous ET-1 was increased in rabbits fed a high-cholesterol diet; this enhanced contractility to ET-1 was completely reversed by L-arginine. These data suggest that L-arginine restores endothelial function and normalizes the synthesis and vasoconstrictor response to ET-1 in hypercholesterolemia.

Subject(s)

Arginine
 Endothelin 1
 Nitric oxide
 Animal experiment
 Animal tissue
 Article
 Atherosclerosis
 Blood vessel reactivity
 Cholesterol intake
 Controlled study
 Endothelium
 Hypercholesterolemia
 Nonhuman
 Priority journal
 Rabbit
 Urinary excretion
 Vasoconstriction
 Animals
 Arginine
 Cholesterol
 Cholesterol, Dietary
 Creatine
 Drug Interactions
 Endothelin-1
 Hypercholesterolemia
 Male
 Metabolic Clearance Rate
 Nitrates
 Rabbits
 Vascular Resistance
 Vasoconstriction

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URI

https://hdl.handle.net/20.500.14740/7231

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Scopus: Year 1983-2021

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