Publication:
Effects of α-mangostin on apoptosis induction of human colon cancer

0
0

Issued Date

2011

Resource Type

Article

File Type

application/pdf

ISSN

10079327

DOI

10.3748/wjg.v17.i16.2086

Other identifier(s)

2-s2.0-79955932364

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

World Journal of Gastroenterology. Vol 17, No.16 (2011), p.2086-2095

Suggested Citation

Watanapokasin R., Jarinthanan F., Nakamura Y., Sawasjirakij N., Jaratrungtawee A., Suksamrarn S. Effects of α-mangostin on apoptosis induction of human colon cancer. World Journal of Gastroenterology. Vol 17, No.16 (2011), p.2086-2095. doi:10.3748/wjg.v17.i16.2086 Retrieved from: https://hdl.handle.net/20.500.14740/7340

Title

Effects of α-mangostin on apoptosis induction of human colon cancer

Author(s)

Watanapokasin R.
 Jarinthanan F.
 Nakamura Y.
 Sawasjirakij N.
 Jaratrungtawee A.
 Suksamrarn S.

Abstract

AIM: To investigate the effect of α-mangostin on the growth and apoptosis induction of human colon cancer cells. METHODS: The three colorectal adenocarcinoma cell lines tested (COLO 205, MIP-101 and SW 620) were treated with α-mangostin to determine the effect on cell proliferation by MTT assay, cell morphology, chro-matin condensation, cell cycle analysis, DNA fragmentation, phosphatidylserine exposure and changing ofmitochondrial membrane potential. The molecular mechanisms of α-mangostin mediated apoptosis were further investigated by Western blotting analysis including activation of caspase cascade, cytochrome c release, Bax, Bid, p53 and Bcl-2 modifying factor.RESULTS: The highest inhibitory effect of α-mangostin on cell proliferation of COLO 205, MIP-101 and SW 620 were 9.74 ± 0.85 μg/mL, 11.35 ± 1.12 μg/mL and 19.6 ± 1.53 μg/mL, respectively. Further study showed that α-mangostin induced apoptotic cell death in COLO 205 cells as indicated by membrane blebbing, chromatin condensation, DNA fragmentation, cell cycle analysis, sub-G1 peak (P < 0.05) and phosphatidylserine exposure. The executioner caspase, caspase-3, the initiator caspase, caspase-8, and caspase-9 were expressed upon treatment with α-mangostin. Further studies of apoptotic proteins were determined by Western blotting analysis showing increased mitochondrial cytochrome c release, Bax, p53 and Bmf as well as reduced mito-chondrial membrane potential (P < 0.05). In addition, up-regulation of tBid and Fas were evident upon treatment with α-mangostin (P < 0.01).CONCLUSION: α-Mangostin may be effective as an anti-cancer agent that induced apoptotic cell death in COLO 205 via a link between extrinsic and intrinsic pathways. © 2011 Baishideng. All rights reserved.

Subject(s)

Alpha mangostin
 Antineoplastic agent
 Caspase
 Caspase 3
 Caspase 8
 Caspase 9
 Cell protein
 Cytochrome c
 Fas antigen
 Garcinia mangostana extract
 Initiator caspase
 Phosphatidylserine
 Plant extract
 Protein Bax
 Protein bcl 2
 Protein Bid
 Protein Bmf
 Protein p53
 Unclassified drug
 Antineoplastic activity
 Apoptosis
 Article
 Cancer cell culture
 Cancer growth
 Cell assay
 Cell cycle
 Cell death
 Cell proliferation
 Cell structure
 Chromatin condensation
 Colon cancer
 Controlled study
 DNA fragmentation
 Drug efficacy
 Drug isolation
 Drug mechanism
 Enzyme activation
 Garcinia mangostana
 Human
 Human cell
 Mitochondrial membrane potential
 Protein expression
 Protein induction
 Protein secretion
 Western blotting
 Apoptosis
 Caspases
 Cell Cycle
 Cell Line, Tumor
 Cell Survival
 Colonic Neoplasms
 Cytochromes c
 DNA Fragmentation
 Enzyme Activation
 Humans
 Protein Kinase Inhibitors
 Xanthones

View online Resources

Research Projects

Organizational Units

Journal Issue

URI

https://hdl.handle.net/20.500.14740/7340

Collections

Scopus: Year 1983-2021

Endorsement

Review

Supplemented By

Referenced By