Publication:
Type I and type II crustins from Penaeus monodon, genetic variation and antimicrobial activity of the most abundant crustinPm4

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Issued Date

2014

Resource Type

Article

File Type

application/pdf

ISSN

0145305X

DOI

10.1016/j.dci.2014.06.015

Other identifier(s)

2-s2.0-84904880634

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Developmental and Comparative Immunology. Vol 47, No.1 (2014), p.95-103

Suggested Citation

Donpudsa S., Visetnan S., Supungul P., Tang S., Tassanakajon A., Rimphanitchayakit V. Type I and type II crustins from Penaeus monodon, genetic variation and antimicrobial activity of the most abundant crustinPm4. Developmental and Comparative Immunology. Vol 47, No.1 (2014), p.95-103. doi:10.1016/j.dci.2014.06.015 Retrieved from: https://hdl.handle.net/20.500.14740/6429

Title

Type I and type II crustins from Penaeus monodon, genetic variation and antimicrobial activity of the most abundant crustinPm4

Author(s)

Donpudsa S.
 Visetnan S.
 Supungul P.
 Tang S.
 Tassanakajon A.
 Rimphanitchayakit V.

Abstract

An antimicrobial protein, crustin, is involved in the innate immunity of crustacean by defending the host directly against the microbial pathogens. By data mining the Penaeus monodon EST database, two type I crustins, carcinin. Pm1 and 2, and ten type II crustins, crustin. Pm1-10, were identified. The abundant crustins were crustin. Pm1, 4 and 7, each with variation in the length of Gly-rich repeat among its members. A few crustin. Pm1, 4 and 7 with deletion in the Cys-rich region were also observed. Furthermore, the crustin. Pm4 with the longest N-terminal Gly-rich region was characterized. The crustinPm4 allelic genes were expressed mainly from the hemocytes. Its expression was up-regulated readily by WSSV infection and gradually decreased to normal level afterwards. The recombinant crustin. Pm4-1 (rcrustin. Pm4-1) isoform was produced using the Escherichia coli expression system and tested for its antimicrobial activity. The rcrustin. Pm4-1 was able to inhibit the growth of a Gram-positive bacterium, Bacillus megaterium but not Bacillus subtilis, Micrococcus luteus and Staphylococcus aureus. It also inhibited the growth of two Gram-negative bacteria, E. coli 363 and Vibrio harveyi 639 at lower potency. The rcrustin. Pm4-1 affected the WSSV infection because the expression of an intermediate early gene ie1 in WSSV-infected hemocyte cell culture was reduced. It was shown further that the rcrustin. Pm4-1 could delay by about one and a half days the manifestation of disease by WSSV. © 2014 Elsevier Ltd.

Subject(s)

Humoral antibody
 Kanamycin
 Recombinant crustinPm4 1 protein
 Recombinant protein
 Thioredoxin
 Type 1 crustin
 Type 2 crustin
 Unclassified drug
 Antimicrobial cationic peptide
 Arthropod protein
 Amino terminal sequence
 Animal cell
 Animal experiment
 Animal tissue
 Antimicrobial activity
 Antiviral activity
 Article
 Bacillus megaterium
 Bacillus subtilis
 Bacterial growth
 Blood cell
 Controlled study
 DNA virus infection
 Drug effect
 Escherichia coli
 Gene expression regulation
 Genetic variability
 Growth inhibition
 LD 50
 Micrococcus luteus
 Nonhuman
 Nucleotide sequence
 Penaeus monodon
 Priority journal
 Protein expression
 Sequence analysis
 Staphylococcus aureus
 Upregulation
 Vibrio harveyi
 White spot syndrome virus
 Amino acid sequence
 Animal
 Cell culture
 Chemistry
 Genetic variability
 Genetics
 Immunology
 Microbiology
 Molecular genetics
 Penaeidae
 Sequence alignment
 Virology
 Amino Acid Sequence
 Animals
 Antimicrobial Cationic Peptides
 Arthropod Proteins
 Cells, Cultured
 Genetic Variation
 Hemocytes
 Molecular Sequence Data
 Penaeidae
 Sequence Alignment

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URI

https://hdl.handle.net/20.500.14740/6429

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Scopus: Year 1983-2021

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