Publication:
Restoration of auditory evoked responses by human ES-cell-derived otic progenitors

2
0

Issued Date

2012

Resource Type

Article

File Type

application/pdf

ISSN

280836

DOI

10.1038/nature11415

Other identifier(s)

2-s2.0-84867337075

Rights Holder(s)

Scopus

Bibliographic Citation

Nature. Vol 490, No.7419 (2012), p.278-282

Suggested Citation

Chen W., Jongkamonwiwat N., Abbas L., Eshtan S.J., Johnson S.L., Kuhn S., Milo M., Thurlow J.K., Andrews P.W., Marcotti W., Moore H.D., Rivolta M.N. Restoration of auditory evoked responses by human ES-cell-derived otic progenitors. Nature. Vol 490, No.7419 (2012), p.278-282. doi:10.1038/nature11415 Retrieved from: https://hdl.handle.net/20.500.14740/6944

Title

Restoration of auditory evoked responses by human ES-cell-derived otic progenitors

Author(s)

Chen W.
 Jongkamonwiwat N.
 Abbas L.
 Eshtan S.J.
 Johnson S.L.
 Kuhn S.
 Milo M.
 Thurlow J.K.
 Andrews P.W.
 Marcotti W.
 Moore H.D.
 Rivolta M.N.

Abstract

Deafness is a condition with a high prevalence worldwide, produced primarily by the loss of the sensory hair cells and their associated spiral ganglion neurons (SGNs). Of all the forms of deafness, auditory neuropathy is of particular concern. This condition, defined primarily by damage to the SGNs with relative preservation of the hair cells, is responsible for a substantial proportion of patients with hearing impairment. Although the loss of hair cells can be circumvented partially by a cochlear implant, no routine treatment is available for sensory neuron loss, as poor innervation limits the prospective performance of an implant. Using stem cells to recover the damaged sensory circuitry is a potential therapeutic strategy. Here we present a protocol to induce differentiation from human embryonic stem cells (hESCs) using signals involved in the initial specification of the otic placode. We obtained two types of otic progenitors able to differentiate in vitro into hair-cell-like cells and auditory neurons that display expected electrophysiological properties. Moreover, when transplanted into an auditory neuropathy model, otic neuroprogenitors engraft, differentiate and significantly improve auditory-evoked response thresholds. These results should stimulate further research into the development of a cell-based therapy for deafness. © 2012 Macmillan Publishers Limited. All rights reserved.

Subject(s)

Cytology
 Hair
 Neurology
 Acoustic nerve fiber
 Article
 Cell differentiation
 Electrophysiology
 Embryonic stem cell
 Evoked auditory response
 Hair cell
 Human
 Human cell
 In vitro study
 Priority journal
 Animals
 Auditory Threshold
 Cell Differentiation
 Cell Line
 Cells, Cultured
 Cochlear Nerve
 Deafness
 Embryonic Stem Cells
 Evoked Potentials, Auditory
 Fibroblast Growth Factor 10
 Fibroblast Growth Factor 3
 Gene Expression Profiling
 Gene Expression Regulation, Developmental
 Gerbillinae
 Hair Cells, Auditory
 Humans
 Mice
 Patch-Clamp Techniques
 Stem Cell Transplantation
 Stem Cells

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https://hdl.handle.net/20.500.14740/6944

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Scopus: Year 1983-2021

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