Publication:
Expressional changes of carbamoyl phosphate synthetase and glutamine synthetase in the liver of rat with thioacetamide-induced cirrhosis

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Issued Date

2013

Resource Type

Article

File Type

application/pdf

ISSN

1252208

Other identifier(s)

2-s2.0-84876853950

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Journal of the Medical Association of Thailand. Vol 96, No.SUPPL.1 (2013), p.S71-S77

Suggested Citation

Khunvirojpanich M., Wattanasirichaigoon S., Pradidarcheep W. Expressional changes of carbamoyl phosphate synthetase and glutamine synthetase in the liver of rat with thioacetamide-induced cirrhosis. Journal of the Medical Association of Thailand. Vol 96, No.SUPPL.1 (2013), p.S71-S77. Retrieved from: https://hdl.handle.net/20.500.14740/6755

Title

Expressional changes of carbamoyl phosphate synthetase and glutamine synthetase in the liver of rat with thioacetamide-induced cirrhosis

Author(s)

Khunvirojpanich M.
 Wattanasirichaigoon S.
 Pradidarcheep W.

Abstract

Background: In order to detoxify ammonia, mammalian livers use carbamoyl phosphate synthetase (CPS) and glutamine synthetase (GS) for conversion into respective non-toxic urea and glutamine. CPS is expressed in the periportal hepatocytes whereas GS is contained in the pericentral hepatocytes. Objective: To examine the expressional changes of CPS and GS in the liver being induced to become cirrhotic by hepatotoxin thioacetamide (TAA). Material and Method: Twenty-five male Wistar rats were divided into 5 groups of 5 animals each. Group 1 was for control. Groups 2 to 5 were treated with 200 mg/kg TAA intraperitoneally three times weekly for 1, 2, 3 and 4 months respectively. The immunohistochemical technique was employed in order to elucidate the expression of CPS and GS in each animal group. Results: As centro-central fibrous bridging developed in the course of TAA treatment, expression of CPS declined dramatically and that of GS was no longer restricted to the pericentral hepatocytes. In month 4, CPS-positive hepatocytes were only found in some regenerative nodules, whereas GS expression became confined to the nodular periphery. Proper CPS staining required tissue fixation in a mixture of methanol, acetone and water (2:2:1 v/v) as opposed to 4% paraformaldehyde. Conclusion: In response to the hepatotoxin TAA, the liver attempts to regenerate by means of conserving persistent CPSpositive hepatocytes and rearranging GS-positive hepatocytes in response to vascular obstruction.

Subject(s)

Carbamoyl phosphate synthase
 Glutamate ammonia ligase
 Thioacetamide
 Alanine aminotransferase blood level
 Alkaline phosphatase blood level
 Animal experiment
 Animal model
 Animal tissue
 Article
 Aspartate aminotransferase blood level
 Controlled study
 Immunohistochemistry
 Liver cell
 Liver cirrhosis
 Liver function test
 Liver regeneration
 Male
 Microscopy
 Nonhuman
 Protein expression
 Rat
 Analysis of Variance
 Animals
 Carbamyl Phosphate
 Glutamate-Ammonia Ligase
 Liver Cirrhosis, Experimental
 Liver Function Tests
 Male
 Rats
 Rats, Wistar
 Thioacetamide

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URI

https://hdl.handle.net/20.500.14740/6755

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Scopus: Year 1983-2021

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