Publication:
Barakol extracted from cassia siamea stimulates chloride secretion in rat colon

2
0

Issued Date

2005

Resource Type

Article

File Type

application/pdf

ISSN

223565

DOI

10.1124/jpet.105.084210

Other identifier(s)

2-s2.0-22944487650

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Journal of Pharmacology and Experimental Therapeutics. Vol 314, No.2 (2005), p.732-737

Suggested Citation

Deachapunya C., Poonyachoti S., Thongsaard W., Krishnamra N. Barakol extracted from cassia siamea stimulates chloride secretion in rat colon. Journal of Pharmacology and Experimental Therapeutics. Vol 314, No.2 (2005), p.732-737. doi:10.1124/jpet.105.084210 Retrieved from: https://hdl.handle.net/20.500.14740/6101

Title

Barakol extracted from cassia siamea stimulates chloride secretion in rat colon

Author(s)

Deachapunya C.
 Poonyachoti S.
 Thongsaard W.
 Krishnamra N.

Abstract

Barakol is a purified extract of Cassia siamea, a plant that has been used as a laxative in traditional medicine. In this study, the effect of barakol on anion transport across the rat colon epithelium was investigated. Colonic epithelium was mounted in Ussing chambers and bathed with Ringer's solution. Addition of 1 mM barakol to the basolateral solution produced a slow increase in short-circuit current (Isc) in proximal colon and distal colon by 24.5 ± 2.2 and 24.2 ± 1.4 μA/cm2, respectively. Barakol increased Isc in a concentration-dependent manner with an EC50 value of 0.4 mM. The barakol-stimulated increase in Isc was inhibited by subsequent treatment with 500 μM diphenylamine-2-carboxylic acid or 400 μM glibenclamide added to the apical solution and 200 μM bumetanide added to the basolateral solution. Pretreatment of the tissues with 200 μM bumetanide, but not 10 μM amiloride, completely abolished the barakol-increased Isc. Ion substitution experiments showed an inhibition of barakol-stimulated Isc in chloride-free solution but not in bicarbonate-free solution. In addition, pretreatment of tissues with 10 μM tetrodotoxin or 10 μM indomethacin, but not 1 μM atropine or 10 μM hexamethonium, partially inhibited the Isc response by barakol. The present results demonstrated the stimulatory effect of barakol on the bumetanide-sensitive chloride secretion in rat colon. The effect of barakol was partly mediated by the stimulation of submucosal nerves and through the release of cyclooxygenase metabolites. These findings thus provide an explanation for the underlying mechanism of barakol as a secretagogue in mammalian colon. Copyright © 2005 by The American Society for Pharmacology and Experimental Therapeutics.

Subject(s)

Amiloride
 Atropine
 Barakol
 Bumetanide
 Chloride
 Efenamic acid
 Glibenclamide
 Hexamethonium
 Indometacin
 Plant extract
 Prostaglandin synthase
 Ringer solution
 Tetrodotoxin
 Unclassified drug
 Animal tissue
 Anion transport
 Article
 Ascending colon
 Chloride transport
 Colon mucosa
 Concentration response
 Descending colon
 Eassia simea
 Intestine secretion
 Male
 Nonhuman
 Priority journal
 Rat
 Senna
 Short circuit current
 Animals
 Anti-Anxiety Agents
 Benzopyrans
 Bicarbonates
 Calcium Channel Blockers
 Cassia
 Chlorides
 Colon
 Cyclooxygenase Inhibitors
 Electrophysiology
 Indomethacin
 Intestinal Mucosa
 Male
 Phenalenes
 Rats
 Rats, Wistar
 Sodium-Potassium-Chloride Symporters
 Stimulation, Chemical

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URI

https://hdl.handle.net/20.500.14740/6101

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Scopus: Year 1983-2021

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