Publication:
Modulatory effects of phytoestrogens on the expression of Fas ligand and the release of cytochrome C in normal and cancerous endometrial cells

0
0

Issued Date

2012

Resource Type

Article

File Type

application/pdf

ISSN

1252208

Other identifier(s)

2-s2.0-84876930108

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Journal of the Medical Association of Thailand. Vol 95, No.SUPPL.12 (2012), p.S105-S112

Suggested Citation

Poonyachoti S., Deachapunya C. Modulatory effects of phytoestrogens on the expression of Fas ligand and the release of cytochrome C in normal and cancerous endometrial cells. Journal of the Medical Association of Thailand. Vol 95, No.SUPPL.12 (2012), p.S105-S112. Retrieved from: https://hdl.handle.net/20.500.14740/6905

Title

Modulatory effects of phytoestrogens on the expression of Fas ligand and the release of cytochrome C in normal and cancerous endometrial cells

Author(s)

Poonyachoti S.
 Deachapunya C.

Abstract

Cytochrome c (CytC) released from mitochondria induces apoptosis in both normal and tumor cells. Expression of Fas ligand (FasL) helps maintain tumor cell survival by inducing apoptosis of Fas-bearing anti-tumor immune cells. A risk of endometrial cancer has been reported to associate with phytoestrogen consumption. Therefore the effects of phytoestrogens, genistein and daidzein, on FasL and CytC protein expression were examined in primary cultured porcine endometrial cells (PE) and human cancerous endometrial cells (RL95-2) by Western blot analysis. Both cells were cultured in standard medium (SM) and switched to estrogen-deprived medium (SF) with or without 17β-estradiol (E, 1 nM), genistein (10 μM) or daidzein (10 μM) for 48 h. FasL (25 kDa) which was found only in RL95-2 cells was upregulated in SF compared to SM. Treatment of RL95-2 cells with E, daidzein or genistein significantly increased the FasL expression by 7-10 folds. In the present study, low level of CytC was detected in both cells cultured in SM but markedly increased in SF by 1.5-2 folds. The SF-induced increase in CytC level was reversed by genistein or daidzein while E suppressed CytC in PE cells, but not in RL95-2 cells. The findings suggest that genistein and daidzein appear to act as a survival factor by inhibiting intracellular apoptogenic initiator in both normal and cancer endometrial cells. In addition, estrogen and phytoestrogens inducing the death signal FasL expressed by cancerous endometrial cells may cause the tumor progression. Thus, consuming phytoestrogen as a supplement should be awareness in patient with endometrial cancer.

Subject(s)

Cytochrome c
 Daidzein
 Estradiol
 Estrogen
 Fas ligand
 Genistein
 Insulin
 Phytoestrogen
 Animal cell
 Apoptosis
 Article
 Controlled study
 Densitometry
 Endometrium cancer
 Human
 Human cell
 Nonhuman
 Protein expression
 Swine
 Upregulation
 Western blotting
 Analysis of Variance
 Animals
 Apoptosis
 Blotting, Western
 Cells, Cultured
 Cytochromes c
 Endometrial Neoplasms
 Estradiol
 Fas Ligand Protein
 Female
 Genistein
 Humans
 Isoflavones
 Phytoestrogens
 Swine

View online Resources

Research Projects

Organizational Units

Journal Issue

URI

https://hdl.handle.net/20.500.14740/6905

Collections

Scopus: Year 1983-2021

Endorsement

Review

Supplemented By

Referenced By