Publication: Detection of the shrimp pathogenic bacteria, Vibrio harveyi, by a quartz crystal microbalance-specific antibody based sensor
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0
Issued Date
2010
Resource Type
File Type
application/pdf
ISSN
9254005
Other identifier(s)
2-s2.0-77149134952
Rights Holder(s)
มหาวิทยาลัยศรีนครินทรวิโรฒ
Bibliographic Citation
Sensors and Actuators, B: Chemical. Vol 145, No.1 (2010), p.259-264
Suggested Citation
Buchatip S., Ananthanawat C., Sithigorngul P., Sangvanich P., Rengpipat S., Hoven V.P. Detection of the shrimp pathogenic bacteria, Vibrio harveyi, by a quartz crystal microbalance-specific antibody based sensor. Sensors and Actuators, B: Chemical. Vol 145, No.1 (2010), p.259-264. doi:10.1016/j.snb.2009.12.003 Retrieved from: https://hdl.handle.net/20.500.14740/7600
Abstract
An immunosensor based on a quartz crystal microbalance (QCM) was developed for detection of Vibrio harveyi (V. harveyi), a pathogenic bacteria causing morbidity and even a high level of fatality in commercial shrimp cultures, including Penaeus monodon. Immobilization of a monoclonal antibody (MAb) against V. harveyi onto the gold electrode of a QCM plate involved a three-step procedure: (1) the formation of a self-assembled monolayer (SAM) of a carboxyl-terminated alkanethiol, (2) activation of the carboxyl groups by N-hydroxysuccinimide (NHS) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), and (3) attachment of the MAb to the activated carboxyl groups. The developed QCM-based immunosensor could be used for the detection of V. harveyi in a working range of 103-107 CFU/mL, with no detectable cross-reactivity to Vibrio vulnificus and Vibrio parahaemolyticus. Controlling the density of surface-immobilized MAb, by the formation of mixed SAMs containing hydroxyl-terminated alkanethiol in combination with the treatment of 1% (w/v) bovine serum albumin (BSA) as a blocking reagent, can significantly improve the binding efficiency of the targeted bacteria. © 2009 Elsevier B.V. All rights reserved.
Subject(s)
Alkanethiols
Binding efficiency
Bovine serum albumins
Carbodiimides
Carboxyl groups
Cross-reactivity
Gold electrodes
Hydroxyl-terminated
Mixed SAM
Monoclonal antibodies (mAb)
N-hydroxysuccinimide
Pathogenic bacterium
Penaeus monodon
Shrimp culture
Vibrio parahaemolyticus
Vibrio vulnificus
Working range
Bacteriology
Body fluids
Chemical detection
Immunosensors
Monoclonal antibodies
Oxide minerals
Piezoelectric devices
Quartz
Quartz crystal microbalances
Self assembled monolayers
Surface plasmon resonance
Bacteria
Binding efficiency
Bovine serum albumins
Carbodiimides
Carboxyl groups
Cross-reactivity
Gold electrodes
Hydroxyl-terminated
Mixed SAM
Monoclonal antibodies (mAb)
N-hydroxysuccinimide
Pathogenic bacterium
Penaeus monodon
Shrimp culture
Vibrio parahaemolyticus
Vibrio vulnificus
Working range
Bacteriology
Body fluids
Chemical detection
Immunosensors
Monoclonal antibodies
Oxide minerals
Piezoelectric devices
Quartz
Quartz crystal microbalances
Self assembled monolayers
Surface plasmon resonance
Bacteria