Publication: Investigation of Transcriptome Profiling in Oryzias uwai (Teleostei: Adrianichthyidae)
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Issued Date
2023
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Scopus
Bibliographic Citation
Science and Technology Asia. Vol 28, No.2 (2023), p.204-215
Suggested Citation
Ngamniyom A., Soe B.K., Wongroj W., Siriwattanarat R. Investigation of Transcriptome Profiling in Oryzias uwai (Teleostei: Adrianichthyidae). Science and Technology Asia. Vol 28, No.2 (2023), p.204-215. Retrieved from: https://hdl.handle.net/20.500.14740/12308
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Abstract
Oryzias is a genus of teleost fish that contains more than 30 recognized species. One species in this genus, O. latipes, is widely used as a model animal in various fields of biological research. However, the transcriptomic profiles are unclear for some species in genus Oryzias. Here, we report transcriptome profiling of freshwater ricefish (Oryzias uwai), wherein RNA sequences of adult O. uwai were analyzed using a BGISEQ-500 platform with gene-annotation analysis. Transcriptomic data showed 58,483 unigenes with a total length, average length, N50, and GC content of 57,148,837, 977, and 1725 bp, and 48.40%, respectively. Unigenes were annotated by alignment using six functional databases: 40,335 from the National Center for Biotechnology Information (NCBI) Protein database, 46,456 from the NCBI Nucleotide database, 34,385 from Swiss-Prot, 29,919 from Eukaryotic Orthologous Groups (KOG), 34,240 from the Kyoto Encyclopedia of Genes and Genomes (KEGG), and 10,050 from Gene Ontology (GO). The numbers of transcriptome coding sequences, transcription-factor coding sequences, and simple sequence repeats (SSRs) were 30,132, 6,184, and 8,306, respectively. To the present body of knowledge, we contribute data from the RNA transcriptome assembly of O. uwai, and our results are of importance for furthering the understanding of gene function in freshwater fish. © 2023, Thammasat University. All rights reserved.