Publication:
Cissus quadrangularis ethanol extract upregulates superoxide dismutase, glutathione peroxidase and endothelial nitric oxide synthase expression in hydrogen peroxide-injured human ECV304 cells

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Issued Date

2012

Resource Type

Article

File Type

application/pdf

ISSN

3788741

DOI

10.1016/j.jep.2012.07.027

Other identifier(s)

2-s2.0-84866041565

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of Ethnopharmacology. Vol 143, No.2 (2012), p.664-672

Suggested Citation

Sapsrithong T., Kaewprem W., Tongumpai S., Nusuetrong P., Meksuriyen D. Cissus quadrangularis ethanol extract upregulates superoxide dismutase, glutathione peroxidase and endothelial nitric oxide synthase expression in hydrogen peroxide-injured human ECV304 cells. Journal of Ethnopharmacology. Vol 143, No.2 (2012), p.664-672. doi:10.1016/j.jep.2012.07.027 Retrieved from: https://hdl.handle.net/20.500.14740/6966

Title

Cissus quadrangularis ethanol extract upregulates superoxide dismutase, glutathione peroxidase and endothelial nitric oxide synthase expression in hydrogen peroxide-injured human ECV304 cells

Author(s)

Sapsrithong T.
 Kaewprem W.
 Tongumpai S.
 Nusuetrong P.
 Meksuriyen D.

Abstract

Ethnopharmacological relevance: Cissus quadrangularis has been widely used in traditional medicine for the treatment of hemorrhoid. However, the detailed mechanism of antioxidant defense of C. quadrangularis in endothelial cells under oxidative stress remains unclear. Aim of the study: The present study aims to elucidate the protective role of ethanol extract of C. quadrangularis (CQE) including its constituents, quercetin and resveratrol, on hydrogen peroxide (H2O2)-injured human umbilical vein endothelial ECV304 cells. Materials and methods: Viability, genotoxicity and protein expression of ECV304 cells were analyzed by MTT, alkaline comet and Western blot, respectively. Production of intracellular reactive oxygen species (ROS) was determined using dichlorofluorescein fluorescence dye. Results: After exposing cells to CQE containing quercetin and resveratrol, DNA damage was not observed. CQE including quercetin and resveratrol significantly attenuated ROS in H 2O2-injured ECV304 cells in a concentration-dependent manner. The protein expression of superoxide dismutase (Cu/Zn-SOD, Mn-SOD), glutathione peroxidase (GPx) and endothelial nitric oxide synthase (eNOS) increased in the cells treated with CQE, quercetin or resveratrol prior to H2O2 exposure, as compared with control. Conclusions: The results provide a molecular mechanism of C. quadrangularis, which could be partially related to quercetin and resveratrol, in restoring ROS in endothelial cells through the upregulation of Cu/Zn-SOD, Mn-SOD, GPx and eNOS. © 2012 Elsevier Ireland Ltd.

Subject(s)

Antioxidant
 Cissus quadrangularis extract
 Dichlorofluorescein
 Endothelial nitric oxide synthase
 Glutathione peroxidase
 Hydrogen peroxide
 Quercetin
 Reactive oxygen metabolite
 Resveratrol
 Superoxide dismutase
 Antioxidant activity
 Article
 Cissus quadrangularis
 Controlled study
 DNA damage
 Genotoxicity
 Human
 Human cell
 Protein expression
 Umbilical vein endothelial cell
 Upregulation
 Western blotting
 Antioxidants
 Cell Line
 Cell Survival
 Cissus
 Comet Assay
 Cytosol
 Ethanol
 Glutathione Peroxidase
 Hemorrhoids
 Human Umbilical Vein Endothelial Cells
 Humans
 Hydrogen Peroxide
 Medicine, Traditional
 Mitochondria
 Nitric Oxide Synthase Type III
 Plant Components, Aerial
 Plant Extracts
 Quercetin
 Reactive Oxygen Species
 Solvents
 Stilbenes
 Superoxide Dismutase
 Cissus quadrangularis

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URI

https://hdl.handle.net/20.500.14740/6966

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Scopus: Year 1983-2021

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