Publication:
Mechanisms of vasorelaxation to gamma-mangostin in the rat aorta

0
0

Issued Date

2012

Resource Type

Article

File Type

application/pdf

ISSN

1252208

Other identifier(s)

2-s2.0-84876937060

Rights Holder(s)

Scopus

Bibliographic Citation

Journal of the Medical Association of Thailand. Vol 95, No.SUPPL.12 (2012), p.S63-S68

Suggested Citation

Tep-Areenan P., Suksamrarn S. Mechanisms of vasorelaxation to gamma-mangostin in the rat aorta. Journal of the Medical Association of Thailand. Vol 95, No.SUPPL.12 (2012), p.S63-S68. Retrieved from: https://hdl.handle.net/20.500.14740/6876

Title

Mechanisms of vasorelaxation to gamma-mangostin in the rat aorta

Author(s)

Tep-Areenan P.
 Suksamrarn S.

Abstract

Objective: To investigate the effects of gamma-mangostin on vascular tone and its mechanisms in the isolated rat aorta. Material and Method: Aortic rings from male Wistar rats were precontracted with methoxamine. Changes in tension were measured using an isometric force transducer and recorded on the MacLab recording system. Vasorelaxant effects of gamma-mangostin were studied in the presence of 300 microM NG-nitro L-arginine methyl ester (L-NAME), 10 microM 1H[1,2,4] oxadiazolo-[4,3-a] quinoxalin-1-one (ODQ), 10 microM indomethacin, 60 mM KCl, 5 mM tetraethylammonium (TEA), 10 microM glibenclamide, 1 mM 4-aminopyridine (4-AP) or 30 microM barium chloride (BaCl2). Moreover,the effects of gamma-mangostin on contraction to CaCl2 were evaluated. Results: Gamma-mangostin (1-100 microM) induced a concentration-dependent vasorelaxation in rat aortic rings precontracted with methoxamine. This effect was significantly reduced after removal of the endothelium and after pre-treatment of the rings with L-NAME, ODQ, high KCl solution, or TEA. However, vasorelaxant responses to gamma-mangostin were not altered by indomethacin, 4-AP, BaCl2 or glibenclamide. Moreover, contractions to CaCl2 (10 mM-30 mM) were reduced by pre-treatment with gamma-mangostin (10 and 100 microM). Conclusion: Gamma-mangostin causes vasorelaxation which is mediated via the NO-cGMP pathway. Moreover, activation of K+ channels and inhibition of extracellular Ca2+ influx from the extracellular space are largely involved in the relaxant effects of gamma-mangostin. These data suggest that gamma-mangostin may acts as an antihypertensive agent.

Subject(s)

1h 1, 2, 4 oxadiazolo [4, 3 a] quinoxalin 1 one
 4 aminopyridine
 Barium chloride
 Calcium chloride
 Carbachol
 Gamma mangostin
 Glibenclamide
 Indometacin
 Methoxamine
 N (g) nitroarginine methyl ester
 Potassium channel
 Tetrylammonium
 Unclassified drug
 Xanthone derivative
 Animal tissue
 Aorta
 Article
 Blood vessel tone
 Calcium transport
 Column chromatography
 Controlled study
 Endothelium
 Garcinia mangostana
 Male
 Nonhuman
 Rat
 Vasodilatation
 Analysis of Variance
 Animals
 Aorta
 Barium Compounds
 Calcium Chloride
 Chlorides
 Endothelium, Vascular
 Glyburide
 Indomethacin
 Male
 Methoxamine
 NG-Nitroarginine Methyl Ester
 Oxadiazoles
 Quinoxalines
 Rats
 Rats, Wistar
 Tetraethylammonium
 Vasodilation
 Vasodilator Agents
 Xanthones

View online Resources

Research Projects

Organizational Units

Journal Issue

URI

https://hdl.handle.net/20.500.14740/6876

Collections

Scopus: Year 1983-2021

Endorsement

Review

Supplemented By

Referenced By