Publication:
Development of a duplex lateral flow dipstick test for the detection and differentiation of Listeria spp. and Listeria monocytogenes in meat products based on loop-mediated isothermal amplification

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Issued Date

2020

Resource Type

Article

File Type

application/pdf

ISSN

15700232

DOI

10.1016/j.jchromb.2019.121834

Other identifier(s)

2-s2.0-85076540014

Rights Holder(s)

มหาวิทยาลัยศรีนครินทรวิโรฒ

Bibliographic Citation

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. Vol 1139, (2020)

Suggested Citation

Ledlod S., Bunroddith K., Areekit S., Santiwatanakul S., Chansiri K. Development of a duplex lateral flow dipstick test for the detection and differentiation of Listeria spp. and Listeria monocytogenes in meat products based on loop-mediated isothermal amplification. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. Vol 1139, (2020). doi:10.1016/j.jchromb.2019.121834 Retrieved from: https://hdl.handle.net/20.500.14740/4652

Title

Development of a duplex lateral flow dipstick test for the detection and differentiation of Listeria spp. and Listeria monocytogenes in meat products based on loop-mediated isothermal amplification

Author(s)

Ledlod S.
 Bunroddith K.
 Areekit S.
 Santiwatanakul S.
 Chansiri K.

Abstract

Listeria spp. are a group of gram-positive bacteria consisting of 20 species. Among them, Listeria monocytogenes is one of the major species that infects humans since it contaminates raw fruits, vegetables, and many others food products. The conventional methods for the detection of Listeria spp. and L. monocytogenes are time-consuming, taking 5–7 days. Herein, a duplex lateral flow dipstick (DLFD) test combined with loop-mediated isothermal amplification (LAMP) was developed for the identification of Listeria spp. and L. monocytogenes within approximately 45 min with the optimized LAMP reaction times at 63 °C. Under the optimized conditions, the method detection limits (MDL) with reference to genomic DNA and pure culture were 900 femtograms (fg) and 20 cfu/mL, respectively. The LAMP-DLFD showed no cross-reactivity with eighteen - other pathogenic bacteria such as Salmonella spp., Staphylococcus aureus, Escherichia coli, Campylobacter coli, C. jejuni, Enterococcus faecalis, Vibrio cholerae, V. parahaemolyticus, Pseudomonas aeruginosa, Shigella dysenteriae, S. flexneri, Bacillus cereus, Lactobacillus acidophilus, L. casei and Pediococcus pentosaceus. Among 100 samples of food products, LAMP-DLFD demonstrated 100% accuracy when compared to other standard detection methods, such as ISO11290-1, enzyme-linked fluorescent assay (ELFA) technology (VIDAS) and PCR. In conclusion, LAMP-DLFD proved to be highly specific and sensitive assays for screening detection of Listeria spp. and L. monocytogenes. © 2019 Elsevier B.V.

Subject(s)

Bacilli
 Bacillus cereus
 Bacteriology
 Diseases
 Escherichia coli
 Food products
 Genes
 Isotherms
 Polymerase chain reaction
 Duplex
 Gram-positive bacterium
 Lactobacillus acidophilus
 Lateral flow dipsticks
 Listeria species
 Loop mediated isothermal amplifications
 Monocytogenes
 Pediococcus pentosaceus
 Listeria
 Genomic DNA
 Protein p60
 Ribosephosphate pyrophosphokinase
 Bacterial DNA
 Article
 Bacillus cereus
 Bacterium detection
 Campylobacter coli
 Campylobacter jejuni
 Controlled study
 DNA extraction
 Duplex lateral flow dipstick test
 Enterococcus faecalis
 Escherichia coli
 Lactobacillus acidophilus
 Lactobacillus casei
 Listeria
 Listeria monocytogenes
 Loop mediated isothermal amplification
 Meat
 Nonhuman
 Pediococcus pentosaceus
 Priority journal
 Pseudomonas aeruginosa
 Salmonella
 Sensitivity and specificity
 Shigella dysenteriae
 Shigella flexneri
 Staphylococcus aureus
 Vibrio cholerae
 Vibrio parahaemolyticus
 Bacterial gene
 Devices
 Equipment design
 Genetics
 Isolation and purification
 Limit of detection
 Listeria
 Listeria monocytogenes
 Meat
 Microbiology
 Nucleic acid amplification
 Pathogenicity
 Procedures
 DNA, Bacterial
 Equipment Design
 Genes, Bacterial
 Limit of Detection
 Listeria
 Listeria monocytogenes
 Meat Products
 Nucleic Acid Amplification Techniques

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URI

https://hdl.handle.net/20.500.14740/4652

Collections

Scopus: Year 1983-2021

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