Publication: Integrating monolithic sorbent micro-solid-phase extraction with anodized screen-printed graphene electrode for serotonin neurotransmitter detection in the presence of ascorbic acid and uric acid
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Issued Date
2026-02-01
Resource Type
ISSN
00263672
eISSN
14365073
Scopus ID
2-s2.0-105027347993
Pubmed ID
41533165
Journal Title
Microchimica Acta
Volume
193
Issue
2
Rights Holder(s)
SCOPUS
Bibliographic Citation
Microchimica Acta Vol.193 No.2 (2026)
Suggested Citation
Thangphatthanarungruang J., Kamsong W., Ar-Sanork K., Ngaosri P., Charonpongsuntorn C., Thaipisuttikul P., Tiyapongpattana W., Chaisuwan P., Karuwan C. Integrating monolithic sorbent micro-solid-phase extraction with anodized screen-printed graphene electrode for serotonin neurotransmitter detection in the presence of ascorbic acid and uric acid. Microchimica Acta Vol.193 No.2 (2026). doi:10.1007/s00604-026-07837-7 Retrieved from: https://hdl.handle.net/20.500.14740/55348
Corresponding Author(s)
Other Contributor(s)
Abstract
A novel analytical method is proposed that integrates monolithic sorbent micro-solid-phase extraction (µ-SPE) with an anodized screen-printed graphene electrode for the determination of serotonin (SER). Monolithic sorbent µ-SPE offers a straightforward and efficient pretreatment method for removing of interfering substances, thereby enhancing the selectivity of SER analysis before being coupled with an electrochemical sensor. Under optimal conditions, the integration of monolithic sorbent µ-SPE with the electrochemical sensor provided a broad linear range of 50–1500 nM for SER detection, with detection and quantification limits of 4.78 and 15.94 nM, respectively. The developed sensor exhibited excellent reproducibility (RSD < 10%) and good stability over a period of 6 months. Furthermore, the proposed method can detect SER even in the presence of ascorbic acid and uric acid at their normal levels in human biological fluids. This method was successfully applied to determine SER levels in real urine samples with an acceptable recovery range of 93.76%–124.14%. The analytical results derived from the developed methodology are strongly correlated with those obtained using the standard high-performance liquid chromatography method.