Publication: Carbon dots prepared from citric acid and urea by microwave-assisted irradiation as a turn-on fluorescent probe for allantoin determination
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Issued Date
2021
Resource Type
Language
eng
File Type
application/pdf
ISSN
11440546
Other identifier(s)
2-s2.0-85121035358
Rights Holder(s)
Scopus
Bibliographic Citation
New Journal of Chemistry. Vol 45, No.47 (2021), p.22424-22431
Suggested Citation
Seedad R., Khuthinakhun S., Ratanawimarnwong N., Jittangprasert P., Mantim T., Songsrirote K. Carbon dots prepared from citric acid and urea by microwave-assisted irradiation as a turn-on fluorescent probe for allantoin determination. New Journal of Chemistry. Vol 45, No.47 (2021), p.22424-22431. doi:10.1039/d1nj03284d Retrieved from: https://hdl.handle.net/20.500.14740/7930
Abstract
This research aimed to develop a simple and selective method for allantoin determination to overcome the limitations of existing colorimetric and alternative methods. A fluorometric detection-based method was developed and used carbon dots (CDs) synthesized from citric acid (CA) and urea as a carbon source. CDs selective for allantoin were synthesized from a mixture of 0.5 g CA and 2.5 g urea exposed to 800 watts of microwave-assisted pyrolysis for 9 minutes. A fluorescence emission spectrum of 438.0 nm was obtained when CDs were excited with a 370.0 nm wavelength. Several detection parameters were investigated including pH, sample: reagent ratio, and reaction time. Under optimum conditions, the proposed method resulted in a detection limit of 0.30 mg L-1, a quantitation limit of 0.98 mg L-1, and a detection range of 1.0-100.0 mg L-1. In summary, the application of this method for measuring the allantoin content of skincare products was demonstrated and accurate and selective detection of allantoin was achieved. Furthermore, a mechanism of fluorescent enhancement of CDs by allantoin was also proposed in this research. © The Royal Society of Chemistry and the Centre National de la Recherche Scientifique.