Publication:
Antifungal Activity and the Chemical and Physical Stability of Microemulsions Containing Citrus hystrix DC Leaf Oil

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0

Issued Date

2020

Resource Type

Article

File Type

application/pdf

ISSN

1934578X

DOI

10.1177/1934578X20957755

Other identifier(s)

2-s2.0-85091078446

Rights Holder(s)

Scopus

Bibliographic Citation

Natural Product Communications. Vol 15, No.9 (2020)

Suggested Citation

Pumival P., Tadtong S., Athikomkulchai S., Chittasupho C. Antifungal Activity and the Chemical and Physical Stability of Microemulsions Containing Citrus hystrix DC Leaf Oil. Natural Product Communications. Vol 15, No.9 (2020). doi:10.1177/1934578X20957755 Retrieved from: https://hdl.handle.net/20.500.14740/4859

Title

Antifungal Activity and the Chemical and Physical Stability of Microemulsions Containing Citrus hystrix DC Leaf Oil

Author(s)

Pumival P.
 Tadtong S.
 Athikomkulchai S.
 Chittasupho C.

Abstract

Citrus hystrix DC (kaffir lime) leaf oil exhibited antifungal activities against Aspergillus niger and Candida albicans. This study aimed to evaluate the antifungal activity of kaffir lime leaf oil and microemulsions containing kaffir lime oil against Trichophyton mentagrophytes var. interdigitale. The chemical components of kaffir lime leaf oil were analyzed by gas chromatography coupled with mass spectrometry. Microemulsions containing kaffir lime oil were formulated using Tween 80, propylene glycol, and water using a phase titration method. The microemulsion of kaffir lime leaf oil was evaluated for droplet size, polydispersity index, and zeta potential using a dynamic light scattering technique. The antifungal activities of kaffir lime oil and its microemulsion were investigated through macrodilution and agar well diffusion methods, respectively. The degradation of citronellal in the microemulsion was analyzed by validated UV-Visible spectrophotometry. The minimum inhibitory concentration value of kaffir lime oil was 1.08 ± 0.00 mg/mL. The microemulsion of kaffir lime leaf oil exhibited potent antifungal activity against T. mentagrophytes var. interdigitale. The size, polydispersity index, and zeta potential of freshly prepared microemulsion were 12.82 ± 0.40 nm, 0.183 ± 0.072, and −7.87 ± 0.06 mV, respectively. The microemulsion of kaffir lime leaf oil also demonstrated good physical and chemical stability at specific temperatures. The kaffir lime oil microemulsion was highly stable when stored at 4 °C and 30 °C for 1 month but was unstable at 45 °C. The microemulsion of kaffir lime leaf oil may be an alternative therapeutic against tinea pedis caused by T. mentagrophytes var. interdigitale. © The Author(s) 2020.

Subject(s)

Antifungal agent
 Bicyclogermacrene
 Caryophyllene
 Caryophyllene oxide
 Citronellal
 Citronellol
 Citronellyl acetate
 Clotrimazole
 Delta cadinene
 Essential oil
 Geranyl acetate
 Hedycaryol
 Humulene
 Isopulegol
 Kaffir lime oil
 Ketoconazole
 Limonene
 Linalool
 Myrcene
 Nerol
 Nerolidol
 Neryl acetate
 Ocimene
 Polysorbate 80
 Propylene glycol
 Sabinene
 Terpinen 4 ol
 Terpinene
 Unclassified drug
 Water
 Antifungal activity
 Article
 Chemical composition
 Citrus
 Citrus hystrix
 Controlled study
 Dispersity
 Drug delivery system
 Gas chromatography
 Hydrodistillation
 Hydrodynamics
 Ionization
 Limit of detection
 Limit of quantitation
 Mass fragmentography
 Mass spectrometry
 Microemulsion
 Minimum inhibitory concentration
 Nonhuman
 Photon correlation spectroscopy
 Plant leaf
 Quantitative analysis
 Titrimetry
 Ultraviolet visible spectrophotometry
 Zeta potential
 Zone of inhibition

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URI

https://hdl.handle.net/20.500.14740/4859

Collections

Scopus: Year 1983-2021

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