Identification of Active Fatty Acid Mixture Extracted from Rice Bran Oil Using NMR Spectroscopy

Abstract

Rice bran oil (RBO) is oil obtained from rice bran. It contains fatty acids and some phytochemicals. RBO has been reported to have some biological activities. Previous publications showed the anticancer activity of rice bran oil fraction extracted from rice bran oil in LoVo, a colorectal cancer cell line through apoptosis via the MAPK pathway with the half-maximal effective concentration (EC50) value of 155.50 ± 1.10 µg/ml. Thus, this study investigated the fatty acid composition of the active rice bran oil fraction purified by column chromatography and identified by1H and13C NMR spectroscopy. The result showed that the active fatty acid fraction consists of saturated fatty acid and unsaturated fatty acid in a ratio of 36:64. These findings may lead to the development of alternative cancer therapies in the future. © 2023 the authors. This work is licensed under the Creative Commons Attribution 4.0 International License.