Abstract:
Contagious wound infection has become one of the most common challenges concomitants with the wounds, causing severe inflammatory responses, and ultimately delaying skin tissue regeneration. Herein, a phenolic-rich Eucalyptus camaldulensis leaf hydrophobic (ECG) and hydrophilic extract (ECY), in varied content was fortified within a hypromellose polymeric matrix was characterized, and further targeted as an effective antioxidative, antimicrobial, anti-inflammatory, and hemostasis dressing. Infrared spectroscopy and thermal analysis of ECG and ECY fortified composite indicated significant hydrogen bonding-based cross-linking, while scanning electron microscopy image showed a porous structure. The chromatography profiling demonstrated 0.022 ± 0.02 and 0.027 ± 0.01 µg/mg of quercetin for the ECG and ECY fortified composite, respectively. The antibacterial and antioxidant activity of extract incorporated composite was significantly (p < 0.001) higher than that of control. Biocompatibility results revealed that composites were compatible with > 80% viability of HaCaT and RAW 264.7 cells. The results of the blood-coagulation and clotting kinetics showed time and dose-dependent hemostasis. Eucalyptus camaldulensis leaf hydrophilic extract incorporated composite significantly (p < 0.001) attenuated the nitrite production against lipopolysaccharides-stimulated macrophage cells. Moreover, the HaCaT cell showed migration of 43.59 ± 1.26 (%) and 48.12 ± 1.85 (%) treated with ECG and ECY incorporated composite after 24 h, respectively. Overall, the hydrophilic extract-incorporated composites showed multifarious biological properties, suggesting their potential for comprehensive wound healing dressing. © 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.