Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/15330
Title: Inter-species differentiation of benign Theilerias by genomic fingerprinting with arbitrary primers
Authors: Chansiri K.
Kawazu S.-I.
Kamio T.
Fujisaki K.
Panchadcharam C.
Watanapokasin Y.
Uthaisang W.
Tananyutthawongese C.
Sarataphan N.
Keywords: animal parasitosis
article
cattle
diagnostic procedure
dna fingerprinting
genome
nonhuman
polymerase chain reaction
random amplified polymorphic dna
species differentiation
theileria
Animals
Asia, Southeastern
Australia
Cattle
DNA Fingerprinting
DNA Primers
DNA, Protozoan
Electrophoresis, Agar Gel
Genome, Protozoan
Japan
Random Amplified Polymorphic DNA Technique
Reproducibility of Results
Species Specificity
Theileria
Theileriasis
Theileria
Issue Date: 1998
Abstract: A method was developed to obtain reproducible DNA fingerprints from five distinct purified benign Theileria genomic DNAs by PCR-based amplification. Randomly amplified polymorphic DNA (RAPD) profiles were obtained from 10 randomly designed 12-mers. However, nine of the 10 primers could generate the difference in RAPD-PCR profiles which allowed discrimination of Theileria species. The method has advantage of being simple, fast and sensitive for diagnosis and characterization of the parasites since it does not require prior DNA sequence information to construct species-specific probes or primers. The results are also beneficial for a proper understanding of the epidemiology and designing rational control programmes for Theileriosis in Asian and South-East Asian countries. Copyright (C) 1998 Elsevier Science B.V.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-0031691332&doi=10.1016%2fS0304-4017%2898%2900155-1&partnerID=40&md5=bd7f506ae3aa345b681218b5854d4915
http://ir.swu.ac.th/jspui/handle/123456789/15330
ISSN: 3044017
Appears in Collections:SCOPUS 1983-2021

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