Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/15237
Title: Effects of ozone treatment on cell growth and ultrastructural changes in bacteria
Authors: Thanomsub B.
Anupunpisit V.
Chanphetch S.
Watcharachaipong T.
Poonkhum R.
Srisukonth C.
Keywords: biocide
ozone
article
Bacillus subtilis
bacterial count
bacterial growth
bacterial membrane
bacteriolysis
bacterium contamination
bacterium culture
cell surface
cell ultrastructure
cell viability
colony forming unit
controlled study
Escherichia coli
exposure
Gram negative bacterium
Gram positive bacterium
membrane rupture
nonhuman
Salmonella
scanning electron microscopy
Staphylococcus aureus
time
water contamination
Bacillus subtilis
Bacillus subtilis
Bacteria (microorganisms)
Escherichia coli
Escherichia coli
Negibacteria
Posibacteria
Salmonella
Salmonella
Salmonella sp.
Staphylococcus
Staphylococcus aureus
Staphylococcus aureus
Issue Date: 2002
Abstract: Ozone appeared to inhibit growth and caused the death of gram negative and gram positive tested bacteria: Escherichia coli, Salmonella sp., Staphylococcus aureus and Bacillus subtilis. Bacterial cultures at 103, 104, 105, 106, and 107 cfu/ml dilution were exposed to 0.167/mg/min/L of ozone at different time intervals (0, 5, 10, 15, 30, 60, 90, 120, and 150 min). Cell viability was observed in all types of tested bacteria at 103, 104, 105 cfu/ml within 30 min after ozone exposure. However, cell inactivation was not significantly observed at concentrations of 106, 107 cfu/ml even after an exposure of 150 min. Ultrastructural changes of treated bacteria showed deformation, rough damage and surface destruction revealed by scanning electron microscopy. Some bacterial cells showed collapsed and shrunken patterns within 60 min and severe rupture and cellular lysis after 90 min of ozone treatment. This study supports the proposed mechanism of the bacteria inactivation by ozone that caused cell membrane destruction and finally lysis reaction. Thus, the precaution of using ozone as a biocide should be used to address appropriate concentrations of bacterial contamination in water.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036444374&doi=10.2323%2fjgam.48.193&partnerID=40&md5=6c1a65cc82007721c6a9211b53fa2cee
http://ir.swu.ac.th/jspui/handle/123456789/15237
ISSN: 221260
Appears in Collections:Scopus 1983-2021

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