Please use this identifier to cite or link to this item: http://ir.swu.ac.th/jspui/handle/123456789/15229
Title: Molecular phylogenetic study of Theileria sp. (Thung Song) based on the thymidylate synthetase gene
Authors: Chansiri K.
Sarataphan N.
Keywords: protozoal protein
thymidylate synthase
protozoal DNA
thymidylate synthase
Babesia bovis
conference paper
controlled study
genetic analysis
nonhuman
nucleotide sequence
phylogenetic tree construction method
priority journal
protozoal genetics
Theileria
theileria buffeli
theileria sergenti
animal
animal parasitosis
article
cattle
cattle disease
classification
DNA sequence
enzymology
genetics
molecular genetics
parasitology
pathogenicity
phylogeny
polymerase chain reaction
Babesia
Babesia bovis
Bos taurus
Theileria
Theileria buffeli
Theileria sergenti
Theileria sp.
Animals
Cattle
Cattle Diseases
DNA, Protozoan
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction
Sequence Analysis, DNA
Theileria
Theileriasis
Thymidylate Synthase
Issue Date: 2002
Abstract: Theileria type Thung Song is an indigenous hemoparasite of dairy cattle from the south of Thailand. It has previously been classified using the analysis of a comparative set of small subunit ribosomal RNA nucleotide sequences. However, the classification of this parasite is still questionable since the Theileria type Thung Song was located as the intermediate parasite between pathogenic and benign groups. We use the thymidylate synthetase gene (TS) as an alternative for the rapid molecular phylogenetic tree construction of benign Theileria type Thung Song, Theileria sergenti and Theileria buffeli with Babesia bovis as an out-group. The partial nucleotide sequences were determined using PCR, cloning and dideoxy sequencing. The TS nucleotide sequence data were aligned and analyzed by distance and maximum likelihood methods to construct the phylogenetic trees. Bootstrap analysis was used to test the strength of the different phylogenetic reconstructions. All tree-building methods gave similar results. This study shows that T. sergenti and T. buffeli are closely related whereas Theileria type Thung Song is more distantly related.
URI: https://www.scopus.com/inward/record.uri?eid=2-s2.0-0036318640&doi=10.1007%2fs00436-001-0567-2&partnerID=40&md5=7fb9d4d4165bed2a3aba94b25f88f9b4
http://ir.swu.ac.th/jspui/handle/123456789/15229
ISSN: 9320113
Appears in Collections:SCOPUS 1983-2021

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